人类疱疹病毒6型与口腔恶性肿瘤相关性的研究

目的：研究人类疱疹病毒6型（HHV-6）的感染与口腔恶性肿瘤的相关性及其在临床诊疗中的意义。方法：运用巢式PCR技术，对40例口腔恶性肿瘤患者的血液、唾液、癌组织、正常组织及加例正常对照人群血液、唾液中人类疱疹病毒6型DNA进行检测分析。结果：恶性肿瘤组和对照组唾液中HHV-6 DNA阳性率分别为85．0％、45．0％，其差别具有统计学意义（P〈0．01）；两组血液中HHV-6检出阳性率分别为67．5％、22．5％，其差别亦具有统计学意义（P〈0．01）。两组唾液中的阳性检出率均大于血液。癌组织阳性检出率（78．95％）显著大于正常组织（10．53％），具有统计学意义（P〈0．01）。结论：进一步证实了唾液腺是HHV-6长期潜伏和增殖的部位，唾液是其主要的传播媒介，为临床简便有效诊断提供参考。HHV-6感染与口腔恶性肿瘤之间存在相关性，可能参与肿瘤发生、细胞癌变的过程。

The Correlation between Human Herpesvirus-6 (HHV-6) and Oral Malignancy

Objective: To investigate the clinical significance of human herpesvirus-6's(HHV-6)infection and its correlation to oral malignancy. Methods: Samples of saliva, peripheral blood mononuclear cells (PBMCs), cancer tissue and normal tissue collected from 40 patients with oral malignancy and 40 healthy young adults were tested for human herpesvirus-6 by nested PCR assays. Results: HHV-6 DNA positive rates were 85.0% and 45.0% in the salivas of patients and control group respectively, 67.5% and 22.5% in the PBMCs of patients and control group respectively, both of which demonstrated statistical difference. In both groups HHV-6 DNA positive rates in saliva were higher than those in PBMCs. The positive rate in cancer tissues(78.95%) was significantly higher than that of normal tissues(10.53%). Conclusion: The result confirms that saliva gland is the latenty and proliferation position of HHV-6 and saliva is the predominant transmission route of HHV-6. The infection of HHV-6 correlates to oral malignancy, which suggests HHV-6 may play a role in the occurrence and cell transformation of malignancy.