Transplantation of dissociated foetal serotonin neurons into the transected spinal cord of adult rats |
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| Authors: | A Privat H Mansour A Pavy M Geffard F Sandillon |
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| Affiliation: | 1. Neurobiologie du Développement, INSERM U-249, Institut de Biologie, F-30406 Montpellier France;2. I.B.C.N., CNRS, Rue Camille St. Saens, F-33077 Bordeaux France;1. Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an Jiaotong University, Xi’an, China;2. Department of Orthopedics, Jining NO.1 People’s Hospital, Jining, China;1. Department of Spine Surgery and Orthopaedic, Xiangya Hospital, Central South University, Changsha, China;2. National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China;1. Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China;2. Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, PR China;3. Department of orthopaedics, Fujian Provincial Hospital, 350001, PR China |
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| Abstract: | Twenty adult rats underwent a complete section of the spinal cord at the lower thoracic level. One week later, 15 of them received a cell suspension obtained from raphe nuclei of 14-day-old foetuses into the distal fragment of the spinal cord. They were sacrificed after survival periods of 10-60 days, and vibratome sections of the spinal cord were processed for immunocytochemical detection of serotonin (5-HT). The control, non-transplanted animals showed a total absence of 5-HT immunoreactivity below the section, whereas the transplanted rats showed many immunoreactive 5-HT perikarya in the graft region, some at a distance of up to 10 mm, and a progressive innervation of the whole grey matter extending at least over 20 mm from the graft site. |
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