| 染料木黄酮对胃癌细胞侵袭转移的抑制作用 |
| |
| 引用本文： | 张俊文,王丕龙.染料木黄酮对胃癌细胞侵袭转移的抑制作用[J].营养学报,2006,28(3):252-254. |
| |
| 作者姓名： | 张俊文 王丕龙 |
| |
| 作者单位： | 重庆医科大学附属第一医院消化内科,重庆,400016 |
| |
| 摘 要： | 目的:研究染料木黄酮(genistein)对胃癌细胞侵袭转移的影响,并初步探讨其作用机制。方法:运用MTT法观察染料木黄酮对人胃癌细胞系SGC-7901细胞粘附能力的影响;用BoydenChamber膜侵袭系统,观察对SGC-7901游走和侵袭的影响;免疫细胞化学法观察对SGC-7901细胞TGF-β1表达的影响。结果:染料木黄酮作用后,SGC-7901细胞粘贴率降低(P<0.05),游走穿膜细胞数(83±4)和侵袭穿膜细胞数(35±3)均明显低于对照组(114±7,60±4,P<0.05),并且能降低TGF-β1的表达。结论:染料木黄酮能有效抑制胃癌侵袭转移。其作用机制可能与通过降低TGF-β1的表达,抑制胃癌细胞异质粘附、运动能力有关。
|
| 关 键 词： | 胃癌细胞 染料木黄酮 侵袭转移 转化生长因子-β |
| 文章编号： | 0512-7955(2006)03-0252-03 |
| 收稿时间： | 04 13 2005 12:00AM |
| 修稿时间： | 2005年4月13日 |
INHIBITION OF GASTRIC CANCER CELLS INVASION AND METASTASIS BY GENISTEIN IN VITRO |
| |
| ZHANG Jun-wen,WANG Pi-long.INHIBITION OF GASTRIC CANCER CELLS INVASION AND METASTASIS BY GENISTEIN IN VITRO[J].Acta Nutrimenta Sinica,2006,28(3):252-254. |
| |
| Authors: | ZHANG Jun-wen WANG Pi-long |
| |
| Abstract: | Objetive: To study the effect of genistein on the invasion and metastasis of human gastric cancer cells SGC-7901 in vitro. Method: MTT was used to study the effects of adhesion by genistein on SGC-7901. The Boyden Chamber membrane invasion culture system was used to study invasion and metastasis. Cell-immunochemistry was used to observe the change of TGF- 1. Results: In the membrane invasion culture system, the numbers of invading and migrating SGC-7901 cells were significantly lower in genistein treated groups than those in the control groups (P<0.05). The adhesion rate and TGF- 1 expression were down-regulated in genistein groups (P<0.05). Conclusion: Genistein can inhibit the migration and invasion of SGC-7901 gastric cancer cells in vitro, probably via down-regulation of TGF- 1. |
| |
| Keywords: | gastric cancer cells genistein invasion and metastasis TGF- 1 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
