miR-10b与乳腺癌侵袭及预后的相关性研究

背景与目的:miR-10b通过增加其下游转移基因RHOC的表达,影响肿瘤的转移和侵袭能力。本研究探讨miR-10b在乳腺癌石蜡组织中的表达及其与乳腺癌侵袭转移及预后的关系。方法:采用实时定量PCR(real-time qPCR)对60例乳腺癌患者原发肿瘤蜡块提取的RNA进行miR-10b表达水平的测定,MannWhitney-U非参数检验比较miR-10b表达水平和乳腺癌临床病理参数的关系,预后分析采用Kaplan-Meier生存分析和log-rank计算。结果:miR-10b表达水平与乳腺肿瘤的大小、淋巴结转移、组织学分级、激素受体状态、肿瘤病理分期、组织类型及是否复发等无明显关系(P>0.05)。Kaplan-Meier生存分析表明,miR-10b表达水平并不影响乳腺癌患者的生存期(P=0.485)。结论:miR-10b表达在乳腺癌侵袭转移中的作用有限,并不是影响预后的主要因素,尚不足以作为基于石蜡组织标本的乳腺癌预测预后指标。

Correlation of miR-10b with invasiveness and prognosis of breast cancer

Background and purpose:The miR-10b has the potential to promote invasiveness and metastasis of tumor by improving the expression of RHOC gene.The objective of this study was to investigate the potential use of miR-10b as a novel breast cancer biomarker.Methods:Extracting RNA from the formalin-fixation and paraffin-embedding(FFPE) breast cancer tissue,and the levels of miR-10b were quantified in specimens of 60 patients with operative breast cancer,by real-time quantitative polymerase chain reaction(RT-qPCR) analysis.The statistical analysis included Mann Whitney-U test,Kaplan-Meier curve and log-rank calculation.Results:There was no statistically significant correlation between miR-10b level and existing clinico pathological parameters of breast cancer,such as tumor size,nodal status,histological grade,hormone receptor status,tumor subtype,stage of disease or relapse result(P>0.05).No correlation was found between miR-10b level and breast cancer survival(P=0.485).Conclusion:It is suggested that the role of miR-10b on invasiveness and prognosis of breast cancer is weak,which limits its potential use as disease classifier and prognostic factor on FFPE tissue.