营心宁对实验性大鼠心肌缺血时PAgT及NO水平的影响

目的 :探讨中药复方制剂营心宁防治冠心病心绞痛的作用机制。方法 :用腹腔注射垂体后叶素 (Pit)法制备心肌缺血缺氧模型 ,观察营心宁及合心爽 (地尔硫唑 )对实验性心肌缺血大鼠一般情况、心电图、血小板聚集率 (PAgT)及心肌组织一氧化氮 (NO)系统水平的变化的影响。结果 :营心宁组和合心爽组大鼠心肌缺血阳性率分别显著低于造模组 (P<0 05) ,且营心宁组和合心爽组间无显著性差异 (P>0 05)。造模组PAgT比阴性对照组显著升高 (P<0 01) ,营心宁组和合心爽组的PAgT显著低于造模组 (分别为P<0 01,P<0 05) ,但仍高于阴性对照组 ,其中营心宁组PAgT低于合心爽组 ,有显著性意义 (P<0 05)。造模组NO含量及NOS活性明显低于阴性组 (P<0 05) ;而营心宁组和合心爽组的NO含量和NOS活性均比造模组明显升高 (P<0 05)。营心宁组NO系统水平低于合心爽组 ,但二者间无显著性差异 (P>0 05)。结论 :营心宁浸膏可降低实验性大鼠心肌缺血心电图阳性率 ,减轻实验性大鼠心肌缺血程度 ,其机制可能为提高心肌组织中NO含量及NOS活性 ,舒张血管平滑肌 ,缓解冠脉痉挛 ,同时降低体内血小板聚集率 ,缓解血液高凝状态 ,防止血栓形成 ,减轻血瘀程度

EFFECT OF Ying Xingning ON PAgT AND NO LEVEL OF EXPERIMENTAL RATS OF MYOCARDIAL ISCHEMIA

Purpose: To study the mechanism of Ying Xinning in treating angina pectoris ( AP ) caused by coronary heart disease. Methods: The myocardial ischemia models were prepared by injecting hypophysin into the abdominal cavity of the rats to observe the effects of Ying Xinning and Herbesser on variations of the general conditions of the experimented rats, their ECG, PAgT and NO system levels. Results: The positive rates of myocardial ischemia in the rats in the Ying Xinning Group and Hebesser Group were lower than those in the model group ( P<0.05 ). But there was no statistical difference between the Ying Xinning and Hebesser Groups ( P>0.05 ). The PAgT in the model group was obviously higher than that in the negative control group( P<0.01 ), while the PAgT in the Ying Xinning Group and Hebesser Group were significantly lower than that in the model group( P< 0.01, P< 0.05 ), but still higher that that in the negative group. The PAgT in the Ying Xinning Group was lower than that in Hebesser Group, with the statistical significance (P<0.05). The NO content and NOS activity in the model group was obviously lower than those in the negative group (P<0.05). However the NO content and NOS activity in the Ying Xinning Group and Hebesser Group were remarkably higher than those in the model group (P<0.05). The NO system level in Ying Xinning Group was lower than that in Hebesser Group with no significant difference between them (P>0.05). Conclusions: Ying Xinning can lower the ECG positive rate of myocardial ischemia in the experimented rats and relieve their ischemic degree. It can also improve NO content and NOS activity of myocardial tissue, dilate vascular smooth muscle, remit coronarospasm, reduce PAgT, prevent thrombosis and relieve blood stasis.