| 脑缺血再灌注后脑内脑源性神经营养因子的基因表达与调节 |
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| 引用本文： | 石向群,陆兵勋,米瑞发.脑缺血再灌注后脑内脑源性神经营养因子的基因表达与调节[J].卒中与神经疾病,2000,7(1):1-3. |
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| 作者姓名： | 石向群 陆兵勋 米瑞发 |
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| 作者单位： | 甘肃兰州市兰州军区总医院神经内科(石向群!730050)，广州南方医院神经内科(陆兵勋)，军事医学科学院三所(米瑞发，范明) |
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| 摘 要： | 探讨：探讨脑缺血再灌注损伤后脑内脑源性神经营养因子（ＢＤＮＦ）ｍＲＡＮ水平的变化，推测ＢＤＮＦ对损伤病理的影响。方法线栓法制作大鼠脑缺血再灌注模，原位交检测大鼠海马神经元内ＢＤＮＦｍＲＮＡ，图像分析间接定量ＢＤＮＦｍＲＮＡ水平。结果（１）脑缺血及缺血再灌注均能诱导双侧海马神经元ＢＤＮＦｍＲＮＡ水平增高；（２）缺血损伤过重后海马神经元ＢＤＮＦｍＲＮＡ水平增高的程度反而小；（３）再灌注后ＢＤＮＦｍＲＮ
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| 关 键 词： | 脑缺血再灌注 海马 基因表达 调节 BDNF |
The brain derived neurotrophic factor mRNA level changes following trainsient focal brain ischemia and reperfusion in brain |
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| Shi Xiangqun,Lu Bingxun,MI Runfa,et al..The brain derived neurotrophic factor mRNA level changes following trainsient focal brain ischemia and reperfusion in brain[J].Stroke and Nervous Diseases,2000,7(1):1-3. |
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| Authors: | Shi Xiangqun Lu Bingxun MI Runfa |
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| Institution: | Shi Xiangqun,Lu Bingxun,MI Runfa,et al.Department of Neurology,Lanzhou Military General Hospital,Lanzhou 730050 |
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| Abstract: | Objective: To study the change of brain derived neurotrophic factor mRNA (BDNFmRNA)in hippocampus and the effect of BDNF on the hippocampus neurons after focal cerebral ischemia and reperfusion in rats.Methods:The level of BDNFmRNA was measured in the rat hippocampus using in situ hybridization.Results :(1)The level of BDNFmRNA in the bilateral hippocampus was increased after cerebral ischemia and reperfusion.(2)The lower level of BDNFmRNA in the hippocampus with serious ischemia was found than that with slight ischemia.(3)The level of BDNFmRNA following the reperfusion was increased continuously.It was the highest at 12 hours and recovered basal level at 72 hours after the reperfusion.Conclusion: The regional basal BDNFmRNA level showed here positively correlated with cellular resistance to ischemic damage,and it was consistent with the hypothesis of a neuroprotective role of BDNF. |
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| Keywords: | Brain derived neurotrophic factor Cerebral ischemia ischemia reperfusion Hippocampus situ hybridization |
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