| HPV16 主要衣壳蛋白L1在昆虫细胞中的表达及免疫学活性分析 |
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| 引用本文： | 孙肖红,周兰萍,孙亚洲,李楠. HPV16 主要衣壳蛋白L1在昆虫细胞中的表达及免疫学活性分析[J]. 免疫学杂志, 2002, 18(4): 273-277 |
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| 作者姓名： | 孙肖红 周兰萍 孙亚洲 李楠 |
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| 作者单位： | 中国医学科学院中国协和医科大学肿瘤研究所,北京,100021 |
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| 基金项目： | 国家“九五”科技攻关课题资助项目 (96 90 6 0 1 11) |
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| 摘 要： | 目的 在昆虫细胞中表达HPV16L1（曲02）蛋白，并分析其免疫学活性。方法 构建表达载体pEASTBACTHb-L1(m202)，用重组病毒感染sf9细胞表达HPV16L1（m202)蛋白，SDS-PAGE，western-blot鉴定其表达，亲和层析和离子交换层析纯化后的产物经鼻腔免疫Balb/c小鼠，竞争抑制ELISA分析免疫血清的中和活性。结果 SDS-PAGE，western-blot结果证明HPV16L1（m202)蛋白的表达，纯化复性后产率约为17%，免疫血清具有竞争抑制HPV中和单抗与HPV16L1（m202)相结合的能力，结论 昆虫细胞表达的HPV16L1（m202)蛋白具有作为预防疫苗的潜力。
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| 关 键 词： | HPV16L1蛋白 昆虫细胞 免疫学活性宫颈癌 HPV16感染 疫苗 |
| 文章编号： | 1000-8861(2002)04-0273-05 |
| 修稿时间： | 2002-02-22 |
Expression of HPV16 L1 protein in insect cells and analysis of its immunoactivity |
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| SUN Xiao hong,ZHOU Lan ping,SUN Ya zhou,LI Nan. Expression of HPV16 L1 protein in insect cells and analysis of its immunoactivity[J]. Immunological Journal, 2002, 18(4): 273-277 |
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| Authors: | SUN Xiao hong ZHOU Lan ping SUN Ya zhou LI Nan |
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| Abstract: | Objective To express and analyse the immune activity of HPV16 L1(m202) protein in insect cells sf9.Methods Expression vector pFASTBAC HTb L1(m202)had been constructed ,sf9 cells were transfected with recombinant viruses and expressed HPV16 L1(m202) protein. The products confirmed by SDS PAGE, western blot, purified by affinity chromatography and ion exchange chromatography, were nasal immunized Balb/c mice, the neutralizing activity of anti serum was analysed by ELISA.Results SDS PAGE and Western blot showed that HPV16 L1(m202) protein had been expressed in sf9 cells, the efficiency of purification and renaturation was approximately 17%, the anti serum has the ability of competing inhibition HPV16 McAb reacting with HPV16 L1(m202) protein.Conclusion HPV16 L1(m202) protein expressed in insect cells has the potentiality of being prophylactic vaccine. |
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| Keywords: | HPV16 L1 protein insect cells immune activity |
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