Human B and T lymphocytes have similar amounts of CD21 mRNA, but differ in surface expression of the CD21 glycoprotein |
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Authors: | Braun M; Melchers I; Peter HH; Illges H |
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Institution: | Division of Rheumatology, Albert-Ludwigs-University, Medical Center, Freiburg, Germany. |
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Abstract: | CD21, the complement receptor type 2 (CR2), binds the complement fragments
iC3b, C3dg and C3d, interacts with CD23 (the low-affinity receptor for
IgE), and binds IFN-alpha. This 145 kDa glycoprotein merits particular
interest because it plays a pivotal role in the activation and
proliferation of B cells by lowering the signal threshold. In human disease
CD21 is important as a receptor for Epstein- Barr virus and HIV. CD21 is
primarily expressed on B lymphocytes and follicular dendritic cells, but
has also been reported on T cells. We established a semi-quantitative PCR
and compared the CD21 mRNA levels of B and T lymphocytes with the
expression of the CD21 glycoprotein on the surface of the respective cells
by flow cytometry. The B cell lines Raji and Ramos and the T cell lines
Jurkat and Molt4 expressed equal amounts of CD21 mRNA, but differed in
surface staining. To address the question to which extent primary human B
and T lymphocytes express CD21 mRNA and membrane-bound CD21 glycoprotein,
we separated B cells, CD4+ and CD8+ T cells from peripheral blood
mononuclear cells of healthy donors. B lymphocytes and CD4+ or CD8+ T cells
expressed similar amounts of CD21 mRNA. Nevertheless only B cells, but not
CD4+ or CD8+ T cells, expressed detectable amounts of CD21 on their cell
surface. Expression of the CD21 exon 11 has been reported being restricted
to follicular dendritic cells only. To the contrary, we found that both
purified B and T cell subpopulations expressed CD21 mRNA with and without
exon 11.
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