牛磺酸拮抗溶血磷脂酸诱导的大鼠血管平滑肌细胞增殖作用

目的　观察牛磺酸 (Tau)对溶血磷脂酸 (LPA)诱导的大鼠血管平滑肌细胞 (VSMC)增殖作用的影响。方法　在培养的大鼠血管平滑肌细胞上 ,测定3 H 胸腺嘧啶核苷(3 H TdR)参入和丝裂素活化蛋白激酶 (MAPK)活性 ;硫代巴比妥酸盐 (TBA)法测脂质过氧化终产物丙二醛 (MDA)含量。结果　LPA (1× 10 -9～ 1× 10 -7mol·L-1)呈浓度依赖性增加VSMC 3 H TdR参入 ,5、10和 2 0mmol·L-1Tau及MAPK抑制剂PD0 980 59预孵育后分别使LPA (1× 10 -7mol·L-1)诱导的3 H TdR参入较单独LPA组减少 15 %、34%、4 8%和 5 4 % (P <0 0 5或P <0 0 1)。LPA增加VSMCMAPK活性 ,5、10和 2 0mmol·L-1Tau预孵育降低LPA(1× 10 -7mol·L-1)刺激的MAPK活性 ,较单纯LPA孵育低14 %、35 %和 4 4 % (P <0 0 5或P <0 0 1)。随着加入LPA的浓度增加 ,细胞MDA含量增加。与 1× 10 -7mol·L-1LPA组比较 ,5、10和 2 0mmol·L-1Tau预处理的细胞较LPA单独孵育组细胞MDA含量分别低 18%、2 7%、4 5 % (P<0 0 5或P <0 0 1) ,LPA诱导细胞MDA生成的作用不受PD0 980 59预孵育的影响 (P >0 0 5 )。结论　LPA呈浓度依赖性促进VSMC增殖和脂质过氧化 ,前者与其细胞内信号传导MAPK途径有关 ,Tau可有效拮抗LPA诱导的VSMC增殖、MAPK激活和脂质过氧化损伤 ,Tau

Taurine inhibits rat vascular smooth muscle cell prolilferation induced by lysophosphatidic acid

AIM To observe effects of taurine on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). METHODS The 3H TdR incorporation and mitogen activated protein kinase (MAPK) activity were measured in cultured VSMC. TBA measured end product of lipid peroxidation MDA. RESULTS LPA enhanced the cultured VSMC 3H TdR incorporation in a concentration dependent manner. LPA increased MAPK activity and MDA content. Taurine (Tau) and PD 098059 inhibited the VSMC 3H TdR incorporation and MAPK activity. Tau inhibited the VSMC MDA content stimulated by LPA, but PD 098059 had no effect. CONCLUSION LPA has a stimulating effect on VSMC proliferation. The LPA induced intracellular signal transduction may be related to MAPK activity. The exogenous Tau can efficiently inhibit VSMC proliferation.