| LACA小鼠骨髓辐射损伤后干细胞因子受体表达的免疫组化研究 |
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| 引用本文： | 彭瑞云,王德文,熊呈琦,李延平,高亚兵,汪宝珍,崔玉芳,杨红,杨瑞彪,崔雪梅. LACA小鼠骨髓辐射损伤后干细胞因子受体表达的免疫组化研究[J]. 中华放射医学与防护杂志, 1998, 18(3): 153-155 |
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| 作者姓名： | 彭瑞云 王德文 熊呈琦 李延平 高亚兵 汪宝珍 崔玉芳 杨红 杨瑞彪 崔雪梅 |
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| 作者单位： | 北京放射医学研究所 |
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| 摘 要： | 目的至今，尚未见到采用免疫组化技术研究骨髓辐射损伤后干细胞因子受体（Ｃ－ｋｉｔ）表达及其在造血重建中的意义。方法经６０Ｃｏγ射线全身照射ＬＡＣＡ小鼠，于照射后４周内分批活杀动物，采用光镜、电镜及ＬＳＡＢ免疫组化方法，研究了辐射诱导的造血细胞凋亡及Ｃ－ｋｉｔ的表达及意义。结果全身照射ＬＡＣＡ小鼠时，５．５Ｇｙ为其亚致死剂量，此剂量照射小鼠可导致外周血白细胞及骨髓有核细胞数减少；骨髓明显损伤及损伤后重建现象；在照后１天内可使骨髓造血细胞发生凋亡；５．５Ｇｙ照射后６小时，Ｃ－ｋｉｔ蛋白于造血细胞浆内明显增多，呈强阳性；照后１０天，骨髓造血细胞增生、恢复，Ｃ－ｋｉｔ蛋白于造血细胞膜表达明显增多，呈强阳性。结论Ｃ－ｋｉｔ表达与造血功能重建有关，在早期造血功能重建中起重要调节作用。
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| 关 键 词： | 辐射 骨髓 细胞凋亡 C-kit 免疫组化 |
| 收稿时间： | 1996-07-15 |
| 修稿时间： | 1996-10-03 |
Immunohistochemical studies on expression of C kit in LACA mouse bone marrow after irradiation |
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| Peng Ruiyun,Wang Dewen,Xiong Chengqi. Immunohistochemical studies on expression of C kit in LACA mouse bone marrow after irradiation[J]. Chinese Journal of Radiological Medicine and Protection, 1998, 18(3): 153-155 |
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| Authors: | Peng Ruiyun Wang Dewen Xiong Chengqi |
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| Affiliation: | Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China;Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China |
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