脑胶质瘤中HIF-1α的表达与凋亡和预后的相关性研究

 目的 本研究旨在探讨缺氧诱导因子-1α(HIF-1α)在脑胶质瘤中的表达与bcl-2、bax及胶质瘤患者预后之间的相关性。方法 采用免疫组织化学SABC法检测HIF-1α、bcl-2、bax表达蛋白在45例胶质瘤及10例正常脑组织中的表达。结果 HIF-1α在正常脑组织中无表达，在45例胶质瘤中28例表达阳性，占62．2％，Ⅰ～Ⅱ级和Ⅲ～Ⅳ级间的表达有显著性差异(P<0．05)。胶质瘤中HIF-1α的表达与bcl-2、bax蛋白表达密切相关(rs=-0．777；rs=0．720，P<0．01)。HIF-1α表达阳性组与阴性组中患者两年生存时间差异有显著性(P<0．05)。结论 HIF-1α表达的异常在胶质瘤的凋亡过程中起重要作用，并与胶质瘤患者的预后密切相关。

The Expression of HIF-1α and Its Relationship to Apoptosis and Prognostic Significance in Human Glioma

Objective 　To explore the expression of HIF-1α inglioma and the relationship between its expression and bcl-2 、bax and Prognostic of patients. Methods 　Expression of HIF-1α bcl-2 and bax was assessed by immunohistochimistry staining in 45 cases of glioma and 10 cases of normal brain tissue. Results 　HIF-1α was expressed in 28 of 45 (62. 2 %) cases of glioma , and not expressed the normal brain tissue. There were significant difference between low grade ( I～II) and high grade ( III～IV) of glioma ( P< 0. 05) . HIF-1α was significantly correlated with expression level of bcl-2 and bax ( rs = - 0. 777 ; rs =0. 720 , P < 0. 01) . There also was a correlation between HIF-1α expression and the two-year survival time ( P < 0. 05) . Conclusion 　The over expression of HIF-1α plays an important role in the apoptosis of glioma. It closely relates with the prognosis of glioma patients.