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Effect of P39 Gene Deletion in Live Brucella Vaccine Strains on Residual Virulence and Protective Activity in Mice |
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| Authors: | Anne Tibor Isabelle Jacques Laurence Guilloteau Jean-Michel Verger Maggy Grayon Valerie Wansard Jean-Jacques Letesson |
| Affiliation: | Laboratoire de Microbiologie et d’Immunologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium,1. and Laboratoire de Pathologie Infectieuse et d’Immunologie, INRA-Tours, Nouzilly, France2. |
| Abstract: | The 39-kilodalton protein (P39) has previously been shown to be an immunodominant protein in Brucella infections. P39 gene deletion mutants of vaccine strains Brucella abortus S19 and Brucella melitensis Rev.1 were constructed by gene replacement. This deletion did not significantly modify the residual virulence of both vaccine strains in CD-1 mice. CD-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. Mutant vaccine strains devoid of P39 could provide a means for differentiating vaccinated from infected animals. |
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