| M-CSF对RAW264.7细胞抗氧化系统的影响 |
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| 引用本文： | 庞战军,周玫,陈瑗.M-CSF对RAW264.7细胞抗氧化系统的影响[J].中国免疫学杂志,2000,16(7):345-348. |
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| 作者姓名： | 庞战军 周玫 陈瑗 |
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| 作者单位： | 第一军医大学生物化学与分子生物学研究所自由基医学研究室,广州 510515 |
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| 基金项目： | 本课题为国家自然科学基金资助项目(编号
39570867,39670197) |
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| 摘 要： | 目的：探讨巨噬细胞集落刺激因子（Ｍａｃｒｏｐｈａｇｅ ｃｏｌｏｎｙ－ｓｔｉｍｕｌａｔｉｎｇ ｆａｃｔｏｒ，Ｍ－ＣＳＦ）抗单核／巨噬细胞氧化损伤的作用机理，揭示Ｍ－ＣＳＦ对小鼠巨噬细胞系ＲＡＷ２６４．７细胞抗氧化系统影响。方法：以Ｌ９２９细胞条件培养液为Ｍ－ＣＳＦ来源，采用酶活笥测定等生化测定方法，考察Ｍ－ＣＳＦ细胞内还原型谷胱甘肽含量、脂质过氧化物含量、过氧化氢酶活性、谷胱甘肽还原酶活性等检测指标
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| 关 键 词： | RAW264.7细胞系 抗氧化酶 动脉粥样硬化 M－CSF |
Effect of macrophage colony-stimulating factor on the antioxidant system in RAW264.7 cells |
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| PANG Zhan-Jun, ZHOU Mei, CHEN Yuan.Effect of macrophage colony-stimulating factor on the antioxidant system in RAW264.7 cells[J].Chinese Journal of Immunology,2000,16(7):345-348. |
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| Authors: | PANG Zhan-Jun ZHOU Mei CHEN Yuan |
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| Abstract: | objective: In order to find out the mechanisms that macrophage colony-stimulating factor (M-CSF) exerted its functions in alleviating the tert-butyl hydroperoxide (tbOOH)-induced oxidative injury to monocytes/macrophages. Methods: M-CSF riched L929 cell conditioned, medium (L929-CM) was used as the source of M-CSF.The antioxidant system in RAW264.7 cells were investigated by applying biochemical analysis such as enzyme activity detection. Results: M-CSF was showed to improve catalase activity and the content of reduced glutathione, while decrease the content of lipoperoxide in RAW264. 7 cells. M-CSF-treated cells also maintained high antioxidant capability when tbOOH attacked. Conclusion: M-CSF could. alleviate the oxidative injury to RAW264 .7 cells through improving their redox status. |
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| Keywords: | Macrophage colony-stimulating factor RAW264 7 cell line Oxidative stress Antioxidant enzymes |
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