The upregulated expression of OX40/OX40L and their promotion of T cells proliferation in the murine model of asthma |
| |
Authors: | Wei Lei Da-Xiong Zeng Can-Hong Zhu Gao-Qin Liu Xiu-Qin Zhang Chang-Guo Wang Qin Wang Jian-An Huang |
| |
Affiliation: | 1.Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; 2.Department of Respiratory Medicine, Children’s Hospital of Soochow University, Suzhou 215003, China; 3.Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China |
| |
Abstract: | ObjectiveTo investigate whether the expression of OX40/OX40 ligand (OX40L) was upregulated in a murine model of asthma and their significance in the pathogenesis of asthma.MethodsAfter an ovalbumin-sensitized/challenged murine model of asthma was established, the expressions of OX40, OX40L in peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage fluid (BALF) cell pellets were measured. Then T cell proliferation was analyzed by cell counting kit-8 (CCK8), and the protein levels of OX40 and OX40L in the lungs were determined by immunohistochemistry. The concentrations of IL-4 and IFN-γ in BALF and T cell culture supernatant were evaluated by ELISA.ResultsThe percentages of CD4+OX40+, CD19+OX40L+, F4/80+OX40L+ in PBMCs and BALF cell pellets were higher in asthma group than in control group (all P<0.01). The proliferation capacity of T cells in asthma group was higher than that in control group (P<0.05). In asthma group, stimulation of OX40 by anti-OX40 mAb obviously promoted T cell proliferation and secretion of IL-4 and IFN-γ. Immunohistochemistry assay showed that OX40 and OX40L protein levels were higher in asthma group than those in control group (all P<0.05).ConclusionsThe expressions of OX40 and OX40L were upregulated in the murine asthmatic model. The upregulation of OX40/OX40L signals could induce the proliferation and cytokines secretion of T cells in asthmatic mice, indicating that OX40/OX40L signal was involved in the pathogenesis of asthma. |
| |
Keywords: | Asthma animal models OX40 OX40 ligand (OX40L) T cell proliferation |
|
|