控制性降压下失血对大鼠海马CA1区细胞凋亡的影响

目的：观察控制性降压下不同程度失血对大鼠海马CA1区细胞凋亡的影响。方法：36只SD大鼠随机分成对照组（C组）和控制性降压组（H组），组内按失血量占全身血容量的百分比不同各分为3组:C1，H1组（10％组）；C2，H2组（20％组）；C3，H3组（30％组）。H组泵入硝普钠复合艾司洛尔控制性降压至平均动脉压50～55 mmHg，稳定10 min后维持此时泵速并开始放血，C 组泵注生理盐水，放血过程同H组，均60 min后复压。用免疫组织化学法检测存活24 h大鼠海马CA1区bcl-2和caspase-3蛋白表达，TUNEL法检测凋亡细胞。结果：Bcl-2和caspase-3的平均光密度值H3组高于C3组(P＜0.05), TUNEL检测的凋亡细胞H3组多于C3组(P＜0.05)。结论：控制性降压下大鼠失血30%可诱导海马CA1区细胞凋亡的发生

Effect of hemorrhage on cell apoptosis in hippocampal CA1 region of rats under controlled hypotension

Objective To explore the effect of hemorrhage on cell apoptosis in the hippocampal CA1 region of rats under controlled hypotension (CH).Methods A total of 36 Sprague Dawley rats were randomly divided into 2 groups: Group C (with no CH) and Group H (with CH).According to different ratios of blood loss to total blood volume, Group C and Group H were redivided into 6 subgroups (6 in each group):C_1,H_1(10%);C_2,H_2(20%);C_3,H_3 (30%). Induced by so-dium nitroprusside and esmolol, the mean aterial pressure in Group H was decreased to 50～55 mmHg and kept for 10 minutes, and then blood loss was started, keeping the pump speed. Without CH, the same style of hemorrhage was performed in Group C. The aterial pressure was increased 60 minutes later after the hemorrhage.Expression of bcl-2 and caspase-3 protein was detected by immunohistochemical method, and apoptosis cells were detected by TUNEL staining. Results The average optical density of bcl-2 and caspase-3 was higher in Group H_3 than that in Group C_3(P＜0.05). There were more apoptosis cells examined by TUNEL in Group H_3 than in Group C_3(P＜0.05).Conclusion Thirty percent blood loss under controlled hypotension can induce cell apoptosis in hippocampal CA1 region in rats.