束缚应激大鼠中枢AMPA受体和相关蛋白mRNA表达的变化及逍遥散对其调节作用

目的观察海马及杏仁核α-氨基羟甲基恶唑丙酸（AMPA）受体各亚基和相关调节蛋白在束缚应激状态下的mRNA表达变化及逍遥散的作用。方法使用捆绑的方法制作束缚应激动物模型，并用逍遥散进行干预，分别于7天后和21天后检测模型大鼠海马CA1区、CA3区、齿状回（DG）、杏仁核AMPA受体亚基GluR1-4、N-乙基顺丁烯二酰亚胺敏感性融合蛋白（NSF）、PKC作用蛋白1（PICKl）mRNA表达的情况。结果7天束缚应激状态下，GIuRlmRNA在海马CA1区表达显著下降（P〈0．05），在CA3区和杏仁核表达显著上升（均P〈0．05），GluR2、GluR3mRNA在杏仁核（均P〈0．05），GluR4mRNA在CA1区（P〈0．01）表达显著增高，NSF、PICK1mRNA表达在杏仁核有明显增高趋势；逍遥散对CA1区GluR4及杏仁核GluRl、GluR2、GluR3mRNA表达变化有显著调节作用（P〈0．05，P〈0．01）。21天束缚应激状态下，CA1区GluR4、DG的GluR2mRNA表达显著下降（均P〈0．05），杏仁核GluRlmRNA表达显著增强（P〈0．01）；逍遥散对CA1区GIuRl、GluR4mRNA表达变化有显著调节作用（均P〈0．05）。结论短期重复应激对于AMPA受体各亚基mRNA表达的影响较强，逍遥散对AMPA受体各亚基mRNA表达的调节在海马CA1区和杏仁核较明显。

Changes of Central AMPA Receptor Subunits and Related Protein mRNA Expression in Immobilization Stressed Rats and Effect of Xiaoyaosan on Them

Objective To observe the changes of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)receptor subunit and related regulatory protein mRNA expression in the hippocampus and amygdala in immobilization stressed rats and effect of Xiaoyaosan(XYS)on them.Methods Immobilization model rats were established by binding for 3 h per day and intervened with XYS,the expression of AMPA receptor subunit (GluR1-4),N-ethylmaleimide sensitive factor (NSF)and protein interacting with C-kinase 1 (PICK1)mRNA expression in model rats' CA1 and CA3 regions of hippocampus,dentate gyrus and amygdala were detected on day 7 and day 21 after modeling.Results On day 7,expression of GluR1 mRNA was significantly decreased in CA1 region (P<0.05)and increased in CA3 region and amygdala (all P<0.05);expression of GluR2 and GIuR3 mRNA in amygdala (all P<0.05 )and GluR4 mRNA in CA1 region (P<0.01)significantly increased, but the expression of NSF and PICK1 mRNA in amygdala only showed an increasing trend.XYS showed effec- tive regulation on GluR4 mRNA in CA1 region (P<0.01 )and GluR1-3 mRNA expression (P<0.05,P<0.01)in amygdala.On day 21,the expression of GluR4 mRNA in CAI region (P<0.05)and GluR2 mRNA in dentate gyrus (P<0.05)markedly lowered and expression of GluR1 mRNA in amygdala increased (P<0.01);XYS significantly regulated the expression of GluR1 and GluR4 mRNA in CA1 region (all P<0.05). Conclusion Repeated stress in a short time shows effect on expression of AMPA receptor subunit mRNA stronger than chronic stress.The regulation of XYS to AMPA receptor subunit mRNA expression were obvious in hippocampal CA1 region and amygdala.