胃扩张模型大鼠迷走神经背核胃相关神经元对不同针刺穴位的反应

背景：课题组的前期工作主要围绕孤束核在接受胃伤害性刺激和针刺穴位反应中所扮演的角色展开研究，而迷走神经背核与孤束核、最后区一起作为迷走神经复合体中不可或缺的组成部分，在调节胃功能中发挥了重要的作用。目的：观察针刺“足三里”、“内关”、“脾俞”、“肾俞”等不同神经节段穴位对胃扩张模型大鼠迷走神经背核胃相关神经元的影响。方法：在成功构建大鼠胃扩张造模的基础上，采用神经电生理学的方法，运用细胞外记录技术，找出迷走神经背核胃相关神经元，予以手针刺激“足三里”、“内关”、“脾俞”、“肾俞”等不同神经节段穴位各15s，记录来自体表的刺激对迷走神经背核胃相关神经元的影响。结果与结论：24只大鼠共记录到90个神经元放电，90个下丘脑室旁核神经元中与胃扩张相关的神经元有54个，54个胃扩张相关神经元中，对针刺足三里、内关、脾俞、肾俞有反应的神经元个数分别为44，39，38，27，有反应的神经元出现比例分别为82%，72%，70%，50%。提示针刺不同神经节段穴位均能不同程度地激活迷走神经背核参与调节胃运动。

Gastric distention-related neurons in dorsal nucleus of the vagus nerve of rats in response to different acupuncture acupoints

BACKGROUND：Preliminary studies of our research group mainly focus on the role of nucleus of solitary tract in gastric noxious stimulation and acupuncture point, while dorsal nucleus of vagus nerve, nucleus of solitary tract and area postrema are the necessary component for vagus nerve complex, and dorsal nucleus of vagus nerve plays a crucial role in the regulation of gastric functions. OBJECTIVE：To observe the effect of acupuncture at Zusanli, Neiguan, Pishu and Shenshu acupoints on the gastric-related neurons in dorsal motor nucleus of the vagus nerve. METHODS：The gastric related neurons in dorsal motor nucleus of the vagus nerve of gastric distension rats were identified using extracellular recording technique, according to neuroelectrophysiology method. Acupuncture stimulation was given to Zusanli, Neiguan, Pishu and Shenshu for 15 seconds. The effect of body stimulation on the gastric distension related neurons in dorsal motor nucleus of the vagus nerve of rats was recorded. RESULTS AND CONCLUSION：Among the involved 24 rats, 90 neurons at paraventricular hypothalamic necleus exhibited discharge. Among the discharged neurons, 54 neurons were related to gastric distension. After acupuncture stimulation at Zusanli, Neiguan, Pishu and Shenshu, there were 44, 39, 38, 27 neurons giving a response, the reaction rate was 82%, 72%, 70%and 50%, respectively. Acupunture at different nerve segment acupoints can activate the dorsal motor nucleus of the vagus nerve to different degrees, which is involved in the regulation of gastric motility.