| 羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性 |
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| 引用本文： | 康雯,;徐国强,;迪丽努尔·阿吉,;魏琴,;马慧,;李兆鹏,;宋倩,;茹菲娅·祖拉提,;阿丽娜·阿布都吉力力,;排黑尔丁·凯赛尔.羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性[J].中国临床康复,2014(34):5466-5472. |
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| 作者姓名： | 康雯 ;徐国强 ;迪丽努尔·阿吉 ;魏琴 ;马慧 ;李兆鹏 ;宋倩 ;茹菲娅·祖拉提 ;阿丽娜·阿布都吉力力 ;排黑尔丁·凯赛尔 |
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| 作者单位： | [1]新疆医科大学第一附属医院,新疆维吾尔自治区乌鲁木齐市830054; [2]新疆医科大学第一附属医院口腔修复科,新疆维吾尔自治区乌鲁木齐市830054; [3]新疆医学动物模型研究实验室自治区重点实验室,新疆维吾尔自治区乌鲁木齐市830054; [4]新疆五家渠市卫生局,新疆维吾尔自治区五家渠市831300 |
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| 基金项目： | 新疆医学动物模型研究重点实验室开放课题项目基金（XJDX1103-2011-08）;新疆维吾尔自治区自然科学基金青年科学基金项目（2013211B59）;新疆医科大学第一附属医院青年科研专项基金（2011QN03） |
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| 摘 要： | 背景：为优化羟基磷灰石的生物活性，前期实验利用等离子喷涂技术在羟基磷灰石表面等离子喷涂制备了压电陶瓷涂层，但此种新型材料的细胞毒性还不明确。目的：评价羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性。方法：将第3代比格犬骨髓间充质干细胞分别接种于羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件上，接种5 d后，扫描电镜观察材料上细胞黏附情况。分别以羟基磷灰石/钛酸钡压电陶瓷试件浸提液、羟基磷灰石试件浸提液、含5%二甲基亚砜和体积分数15%胎牛血清的低糖DMEM溶液、只含体积分数15%胎牛血清的低糖DMEM培养基培养第3代比格犬骨髓间充质干细胞，培养的1，3，5 d采用CCK-8法检测各组细胞毒性。结果与结论：骨髓间充质干细胞在羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件表面增殖旺盛，达到复层生长，伪足与细胞之间连接很紧密，胞体丰富，表明两组材料具有良好的细胞相容性。CCK-8细胞毒性实验显示，羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件组细胞相对增殖率均在80%以上，毒性分级为1级，无细胞毒性。
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| 关 键 词： | 生物材料 材料相容性 钛酸钡 压电陶瓷 细胞毒性 生物相容性 CCK-8 等离子喷涂 骨髓间充质干细胞 羟基磷灰石 扫描电子显微镜 新疆维吾尔自治区自然科学基金 |
In vitro cytotoxicity of the hydroxyapatite/barium titanate biological piezoelectric ceramic coating |
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| Institution: | Kang Wen, Xu Guo-qiang, Diilnur Aji, Wei Qng, Ma Hui, Li Zhao-peng, Song Qian, Ruflya Zulati, Alina Abdujilil, Pahirdin Kaisar(1 First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 2.Department of Prosthodontics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 3Xinjiang Laboratory of Animal Models (Key Laboratory of Xinjiang UygurAutonomous Region), Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 4Health Bureau of Wujiaqu, Wujiaqu 831300, Xinjiang Uygur Autonomous Region, China) |
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| Abstract: | BACKGROUND：In order to optimize the biological activity of hydroxyapatite, previous experiments have used plasma spraying technique to prepare a piezoelectric ceramic coating on the surface of hydroxyapatite, but the cytotoxicity of this new material is not clear. OBJECTIVE： To evaluate the cytotoxicity of hydroxyapatite/barium titanate biological piezoelectric ceramic coatingin vitro. METHODS： The 3rd generation beagle bone marrow mesenchymal stem cels were seeded on hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens, respectively. After 5 days, the celladhesion was detected by scanning electron microscopy. The 3rd generation bone marrow mesenchymal stem cels were also co-cultured with hydroxyapatite/barium titanate piezoelectric ceramic specimen extract, hydroxyapatite specimen extract, low-glucose Dulbecco’s modified Eagle’s medium containing 5% dimethylsulfoxide and 15% fetal bovine serum, and low-glucose Dulbecco’s modified Eagle’s medium containing 15% fetal bovine serum, respectively. The cytotoxicity was tested by cellCounting Kit-8 assay at days 1, 3, 5 after co-culture. RESULTS AND CONCLUSION：Bone marrow mesenchymal stem cels on the surface of hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens grew proliferatively and presented with multi-layer growth. The connection between cels and pseudopodia was very close, which indicates that the two kinds of materials both have good cytocompatibility. cellCounting Kit-8 assay showed that the cels cultured in the extracts of hydroxyapatite/barium titanate biological piezoelectric ceramic and hydroxyapatite specimens proliferated more than 80%, and the toxicity was grade 1 that meant no cytotoxicity. |
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| Keywords: | ceramics materials testing hydroxyapatites |
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