HLA-A,B,C抗原在原发肺癌及其淋巴结转移灶中的表达

目的：研究肺癌及其淋巴结转移灶中人类白细胞抗原(HIA—A，B，C)的表达情况，并探讨其临床意义。方法：用抗HIA—A，B，C单克隆抗体W6/32，，以免疫组化S—P法检测67例肺癌和23例肺癌淋巴结转移灶中HIA—A，B，C抗原的表达。结果：6例小细胞肺癌，27例鳞癌和34例腺癌中HIA—A，B，C抗原表达下降率分别为100％，66．6％和38．2％，后两者相比差异有统计学意义(P＜0．05)；非小细胞肺癌中随着肿瘤分化程度下降，HIA—A，B，C抗原缺失率将增加(P＜0．05)，而与病人的TN分期无关；转移灶中HIA—A，B，C抗原的表达与其原发灶中相比普遍减低(P＜0．05)。结论：HIA—A，B，C抗原在肺癌中的表达明显低于正常肺组织；且与肺癌的组织类型和分化程度相关。

Expression of Human Leucocyte Antigen-A, B, C in Primary Lung Carcinomas and Metastatic Lymph Nodes

Objective: Our aims were to study the expressions of human leucocyte antigen (HLA-A,B,C) in primary lung carcinomas and the metastatic focus and to discuss their clinical significance. Methods: Using the monoclonal antibody against HLA-A,B,C antigens ( w6/32) , we detected the expression of human leucocyte antigen in 67 primary lung tumors and 23 metastatic focus through the immunohistochemical S-P method. Results: The declension rate of the expression of HLA-A,B,C in small cell carcinoma was 100% , in squamous cell carcinoma 66.6% , and in adenocarcinoma 38. 2%. The discrepancy between these results was of statistical importance (P < 0. 05). The declension rate of HLA-A, B, C antigens increased with the decreasing of tumor's differentiation in NSCLC ( P < 0. 05 ) , but there was no significant relation between the rate and TN stage of the patients. The expression of HLA-A, B, C antigen in metastatic focus were more deficient than those in the primary tumors (P < 0. 05). Conclusion: The expression of HLA-A,B,C antigen is obviously lower in lung cancer than that in normal lung, and is correlated with tumors tissue type and differentiation degree.