P物质抗体对豚鼠耳蜗核下丘核团内听觉诱发电位调谐曲线的影响

探索Ｐ物质在听觉脑干中枢中对声信号的频率分析作用。方法采用短音刺激，短纯音前掩蔽法和豚鼠耳蜗核、正丘、核团内电极，记录ＣＮ及ＩＣ核团内听觉诱发电位。观察核团内注射微量Ｐ物质抗体或对照注射等量兔血清后ＣＮ和ＩＣ核团内听觉诱发电位调谐曲线的变化。

The effects of substance P antibody on tuning curve of auditory evoked potential of cochlear nucleus and inferior colliculus in guinea pigs]

OBJECTIVE: To explore the effects of substance P on the frequency analysis of sound signals in the auditory brainstem. METHODS: The electrodes were implanted into the cochlear nucleus (CN) and the inferior colliculus (IC) of guinea pigs. The auditory evoked potential in CN (CN-AEP) and IC (IC-AEP) was recorded with tone pip stimulation and tone burst forward masking techniques. The Q10 dB values of the tuning curves were compared between the CN-AEP and IC-AEP after injection of SP antibody or rabbit serum with the same dosage (used as control) into the nucleus. RESULTS: After injection of SP antibody, the Q10 dB values of the tuning curves of CN-AEP and IC-AEP had no significant changes in 1 kHz and 2 kHz probe tone groups. When probe tone frequencies were 4 kHz and 6 kHz, there was a significant difference between the two frequency groups in Q10 dB. In addition, the higher frequency slope and lower frequency slope value had corresponding changes. CONCLUSION: The results imply that SP could act as a neuromediator in the auditory afferent system and participated in the high frequency analysis in the auditory brainstem.