猪囊尾蚴cC1亚单位疫苗毕赤酵母高表达体系的优化

目的　优化猪囊尾蚴 c C1亚单位疫苗毕赤酵母表达系统。方法　毕赤酵母转化菌在 BMMY、BMM、MM及各加 1%酪蛋白水解物 (CA)的诱导培养基中经 0 .5 %甲醇诱导表达后 ,上清液行 SDS- PAGE。结果　高拷贝毕赤酵母转化菌 SMD116 8的表达水平高于酵母转化菌 GS115 ;Mut S型比 Mut+型表达量高 ;表达水平与拷贝数呈正相关 ,即拷贝数越高 ,表达量也越高 ;在诱导培养基 BMMY中的表达水平明显高于其它诱导培养基 ;阴性对照菌未见目的表达条带。结论　猪囊尾蚴 c C1亚单位疫苗在毕赤酵母高表达系统中得到优化 ,为大量制备生产打下了基础。

To Obtain high-level expression of recombinant cysticercus cellulosae Antigen cC1 in methylotrophic yeast pichia pastoris

Objective To obtain high level expression of Cysticercus cellulosae antigen cC1 in mythylotrophic yeast Pichia pastoris.Methods High copy number transformants of Pichia pastoris were induced by methanol in induced media BMMY,BMMY CA,BMM,BMM CA and MM,MM CA for the expression of r cC1.Supernatants after induction were analyzed by SDS PAGE.Results The results revealed that high copy number transformants of Pichia pastoris yeast SMD 1168 had a higher expression than high copy number transformants of Pichia pastoris yeast GS115.Mut s transformants of high copy number gave higher level expression in BMMY.Conclusion Cysticercus cellulosae antigen cC1 has been highly expressed in mythylotrophic yeast Pichia pastoris and it has laid the foundation of large scale production.