二甲基亚砜和珍黄注射液联合用药对小鼠宫颈癌的抗癌作用

目的：探讨二甲基亚砜（Dimethl Sulphoxide,DMSO)和珍黄注射液（Zhenhuang injection,ZHI)联合用药对小鼠宫颈癌的抗癌作用，为临床试验提供科学依据。方法：用DMSO（50mg/kg)，ZHI（20mg/kg),DMSO ZHI(DMSO 50mg/kg,ZHI 20mg/kg)分别对小鼠宫颈癌U14腹水瘤模型和实体瘤模型进行治疗，以生命延长率和抑瘤率作为疗效评价指标。实验重复两次，结果：在腹水瘤治疗实验，DMSO组，ZHI组，DMSO＋ZHI组的生命延长率分别为21．37％－25．86％，41．38％－43．59％，65．81％－69．83％，经统计学分析，三个治疗组与对照组比较均有极显著性差异（P＜0．01），联合用药组（DMSO＋ZHI组）与单药组（DMSO组或ZHI组）比较均有极显著性差异（P＜0．01），在实体瘤治疗实验，DMSO组，ZHI组，DMSO＋ZHI组的抑瘤率分别为22．50％，25．83％，37．50％，41．67％，60．83％，68．33％，经统计学分析，三个治疗组与对照组比较均有显著或极显著性差异（P＜0．05或P＜0．01），联合用药组与单药组比较均有极显著性差异（P＜0．01），结论：ZHI对小鼠宫颈癌U14有一定的抗癌作用，DMSO和ZHI联合用药能起增效作用。

Antitumor effect of combination of dimethl sulphoxide and Zhenhuang injection on cervical carcinoma of mice

Objective: To explore the antitumor effect of combination of dimethl sulphoxide (DMSO) and Zhenhuang injection (ZHI) on cervical carcinoma of mice so as to provide scientific dasis for clinical experiments. Methods: The model of ascitic tumor and the model of solid tumor of cervical carcinoma U14 were treated with DMSO(50mg/Kg), ZHI(20mg/Kg) and DMSO with ZHI(DMSO 50mg/Kg, ZHI 20mg/Kg), respectively.The efficacy estimation indices of the test included the life-prolonging rate and the tumor-inhibiting rate.The experiment was repeated two times. Results: The treatment to ascitic tumor, the life-prolonging rates were 21.37%～25.86%, 41.38%～43.59%, and 65.81%～69.83% in DMSO group, ZHI group and in group of combination of DMSO and ZHI, respectively. Through statistic analysis, there was significant difference between the treated groups and control group(P<0.01). There was marked difference in group of combination of DMSO and ZHI, compared with DMSO group or ZHI group(P<0.01). The treatment to solid tumor, the tumor-inhibiting rates were 22.50%～25.83%, 37.50%～41.67%,and 60.83%～68.33% in DMSO group, ZHI group and in combined group, respectively. Through statistical analysis, there was remarkable or very remarkable difference between treated groups and control group(P<0.05 or P<0.01).There was marked difference in the combined group, compared with single treatment group(P<0.01). Conclusions: ZHI had certain antitumor effect on cervical carcinoma U14 of mice. Combination of DMSO and ZHI might produce additive effect.