黄连素对人肝癌Hep G_2细胞周期及凋亡的影响

目的:观察黄连素对人肝癌细胞Hep G2生长的影响及其可能机制。方法:将浓度分别为0,25,50,100,200μmol/L的黄连素与人肝癌细胞Hep G2共同培养24,48,72 h,用RSB法检测黄连素对人肝癌细胞HepG2生长的抑制率;用流式细胞分析仪分析细胞周期的变化、检测细胞凋亡率及Caspase-3、CyclinB1、CDC2、CDK4蛋白的表达。结果:黄连素对人肝癌细胞Hep G2生长抑制率随药物浓度和作用时间的增加而增大;流式细胞仪分析显示黄连素将人肝癌细胞Hep G2阻滞于S、G2/M期。黄连素作用48 h后人肝癌细胞Hep G2凋亡率明显增加,且随药物浓度的增加而加大。黄连素作用48 h后Hep G2细胞的Caspase-3、CDC2蛋白表达增加;而CDK4、Cy-clinB1蛋白表达降低。结论:黄连素可能通过增加Hep G2细胞CDC2蛋白表达,降低CDK4、CyclinB1蛋白表达使其阻滞于S、G2/M期;可能通过增加Caspase-3蛋白表达增加Hep G2细胞凋亡。

Berberine Induces Cell Cycle Arrest and Apoptosis in Human Hepatocelluar Carcinoma Hep G2 Cell Line

Objective: To study the inhibiting effects of berberine on human hepatocelluar carcinoma cell line HepG2 and the molecular mechanism.Methods: HepG2 cells were incubated with berberine at various concentration and time,cell growth inhibition was assessed by Sulphorhodamine assay,cell-cycle and apoptosis were analyzed by flow cytometry.Caspase-3,CyclinB1,CDC2 and CDK4 proteins expressions were also assessed by flow cytometry.Results: The growth of HepG2 cells was significantly inhibited by berberine in a dose-dependent and time-dependent manner.A concentration-dependent decrease of cell in G0/G1 phase and increase in S and G2/M phase were detected which was associated with an increased expression of CDC2 proteins and decreased expression of Cyclin B and CDK4 proteins.A concentration-dependent increase of cell apoptosis was detected which was associated with a marked increment of the expression of Caspase-3 proteins.Conclusion: Berberine may arrest HepG2 cells in S and G2/M phase of cell cycle through inhibiting CyclinB and CDK4 and increasing CDC2 expression.Berberine may induce HepG2 cells apoptosis through increasing the level of Caspase-3.