人胚胎生长发育过程中Bcl-2和Bax蛋白在食管组织中的表达

目的探讨人胚胎食管发育过程中食管壁细胞增殖与凋亡的变化规律,以及B细胞淋巴瘤/白血病-2(Bcl-2)和Bcl相关X蛋白(Bax)在食管生长发育中的表达意义。方法应用免疫组织化学ABC法检测第2、3、4月龄段人胚胎(共14例)食管壁组织内Bcl-2和Bax蛋白的表达。结果第2、3、4月龄段,人胚胎食管壁肌间神经丛、黏膜下神经丛和黏膜层内均有Bcl-2蛋白阳性表达,在食管上皮Bax蛋白呈弱阳性表达。结论Bcl-2和Bax细胞周期调控蛋白与人胚胎食管的生长发育关系密切。

EXPRESSION OF Bcl-2 AND BAX PROTEINS IN THE DEVELOPING ESOPHAGUS OF HUMAN EMBRYO

Objective To explore the rule of cell proliferation,apoptosis and expression significance of involved proteins as Bcl-2 and Bax in the developing esophagus of human fetus. Methods In the second through the fourth month of gestation,the expressions of Bcl-2 and Bax proteins were observed in 14 human esophagus using the ABC of immunohistochemical methods. Results In the second,the third and the fourth months of gestation,there were Bcl-2 positive cells distributed in the intermuscular and submucous nerve plexus,and the mucous epithelial cells in the human esophagus.Bax weakly positive cells were observed in the mucous epithelial cells of the human esophagus.Conclusion Bcl-2 and Bax proteins are closely related with the developing esophagus of human embryo.