| 食品中单核细胞增生李斯特菌PCR快速检测方法 |
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| 引用本文： | 徐德顺,沈月华,俞明华. 食品中单核细胞增生李斯特菌PCR快速检测方法[J]. 现代预防医学, 2007, 34(16): 3040-3041 |
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| 作者姓名： | 徐德顺 沈月华 俞明华 |
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| 作者单位： | 湖州市疾病预防控制中心,湖州,313000 |
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| 基金项目： | 湖州市医药卫生科学研究计划项目 |
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| 摘 要： | [目的]建立食品中单核细胞增生李斯特菌的快速、敏感、特异的PCR检测方法。[方法]选择Hly基因作为靶序列设计一对引物，用该引物对单增李斯特菌和10株非单增李斯特菌进行PCR扩增，并且用此方法对30份食品进行检测。[结果]扩增片断表现出极好的单增李斯特菌特异性，最低检出限为190cfu/ml。[结论]本实验建立的PCR检测方法为一种简便、快速、敏感、特异的单增李斯特菌检测方法。
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| 关 键 词： | 食品 单核细胞增生李斯特菌 聚合酶链反应 |
| 文章编号： | 1003-8507（2007）16-3040-02 |
| 收稿时间： | 2006-10-16 |
| 修稿时间： | 2006-10-16 |
DEVELOPMENT OF A RAPID, SENSITIVE AND SPECIFIC POLYMERASE CHAIN REACTION (PCR) METHOD FOR DETECTION OF LISTERIA MONOCYTOGENES (LM) IN FOODS |
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| XU De-shun,SHEN Yue-hua,YU Ming-hua. DEVELOPMENT OF A RAPID, SENSITIVE AND SPECIFIC POLYMERASE CHAIN REACTION (PCR) METHOD FOR DETECTION OF LISTERIA MONOCYTOGENES (LM) IN FOODS[J]. Modern Preventive Medicine, 2007, 34(16): 3040-3041 |
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| Authors: | XU De-shun SHEN Yue-hua YU Ming-hua |
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| Affiliation: | Huzhou Municipal Center for Disease Control and Prevention, Huzhou 313000,China |
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| Abstract: | [Objective]To establish a rapid,sensitive and specific polymerase chain reaction(PCR)method for detection of Listeria monocytogenes(LM)in food.[Methods]A pair of oligonucleotide primers was designed with hlyA gene as target sequence. Using the designed primers,LM and 10 strains of other bacteria were amplified by PCR,and the method was used to detect LM in 30 food samples.[Results]Amplified fragments showed excellent features of LM. The detection Limit of the method was 190cfu/ml in pure cultured broth.[Conclusions]The PCR technique is a simple,rapid,sensitive and specific method for detection of LM. |
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| Keywords: | Food Listeria monocytogenes Polymerase chain reaction |
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