| 应用DNA探针检测蚊体内疟原虫的研究 |
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| 引用本文： | 刘克义,陈锡欣,黄炳成,程义亮, 蔡贤铮,梁泽堂,林世干. 应用DNA探针检测蚊体内疟原虫的研究[J]. 中国病原生物学杂志, 1994, 0(2) |
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| 作者姓名： | 刘克义 陈锡欣 黄炳成 程义亮 蔡贤铮 梁泽堂 林世干 |
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| 作者单位： | 山东省寄生虫病防治研究所，海南省热带病防治研究所 |
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| 摘 要： | 本文用生物素标记的克隆ＤＮＡ探针，以斑点杂交法检测蚊体内恶性疟原虫。将一组蚊虫在还原性缓冲液中研磨后集体检测时，在２５只蚊中有１只感染蚊即可检出，亦可将单个蚊虫直接压在硝酸纤维素膜上进行检测。本技术的高特异性、敏感性以及试剂的稳定性等表明，它可作为一个适用的流行病学调查工具。
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| 关 键 词： | 恶性疟原虫，DNA探针，大劣按蚊 |
DETECTION OF MALARIA PARASITES IN MOSQUITO WITH DNA PROBE |
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| Liu Ke-Yi Chen Xi-Xin Huang Bing-Cheng Cheng Yi-Liang Shandong Institute of Parasitic Diseases. DETECTION OF MALARIA PARASITES IN MOSQUITO WITH DNA PROBE[J]. Journal of Pathogen Biology, 1994, 0(2) |
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| Authors: | Liu Ke-Yi Chen Xi-Xin Huang Bing-Cheng Cheng Yi-Liang Shandong Institute of Parasitic Diseases |
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| Affiliation: | Jining 272133 |
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| Abstract: | This paper reports the result of detection of P.f.in mosquito with Dot hybridiza-tion using 32P-labelled cloned DNA probe.The minimal detectable limit was 1 infected mosquitoout of every 25 mosquitos. It is highly recommendable to grind a group of mosquitos in reducedbuffer for detection of large samples,while highly advisable to crush a single mosquito onto nitro-cellulose paper for accurate estimation of sporogony.The specificity,sensitivity,simplicity aswell as the stability of the reagents suggested that this technique could be ideally used as a toolfor epidemiological investigation of malaria. |
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| Keywords: | Plasmodium falciparum DNA probe An.dirus |
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