| 宋内氏福氏2a双价志贺氏菌芳香族氨基酸营养缺陷型减毒株的生物学特性 |
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| 引用本文： | 刘士辉,毛培基,牟兆钦. 宋内氏福氏2a双价志贺氏菌芳香族氨基酸营养缺陷型减毒株的生物学特性[J]. 中华微生物学和免疫学杂志, 1994, 0(3) |
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| 作者姓名： | 刘士辉 毛培基 牟兆钦 |
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| 作者单位： | 军事医学科学院微生物流行病研究所，北京军事医学科学院生物工程研究所 |
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| 摘 要： | ＦＳ５４－２、ＦＳ５４－３、ＦＳ５４－４、ＦＳ５４－５、Ｐｓ５４－６、ＰＳ５４－７及ＦＳ５４－７ｂ等株为本室构建的宋内氏福氏２ａ双价志贺氏菌芳香族氨基酸营养缺陷型减毒株，不但其Ｔｎ１０所携带的四环素抗性已消除，ＦＳ５４－７ｂ株的Ⅰ相大质粒Ｔｎ５所携带的卡那霉素抗性亦已消除。本文为在此基础上，进一步对双价减毒株的侵入力、毒力、免疫原性与免疫保护性及遗传稳定性等进行的研究，证明双价减毒株仍保持了入侵肠上皮细胞的能力，动物实验安全低毒，有很好的免疫原性，对小白鼠有很好的双重免疫保护性。此株显示了良好的应用前景。
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| 关 键 词： | 减毒株；芳香族氨基酸营养缺陷型；侵袭肠上皮细胞能力 |
STUDY ON SOME BIOLOGICAL PROPERTIES OF THE STRAIN OF FS54-7b(AN AROMATIC ACID AUXOTROPHIC ATTENUATED BIVALENT HYBRID STRAIN OF S. SONNEI AND S. FLEXNERI 2a |
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| Liu Shi-hui,Mao Pei-ji,Mu Zhao-qin. STUDY ON SOME BIOLOGICAL PROPERTIES OF THE STRAIN OF FS54-7b(AN AROMATIC ACID AUXOTROPHIC ATTENUATED BIVALENT HYBRID STRAIN OF S. SONNEI AND S. FLEXNERI 2a[J]. Chinese Journal of Microbiology and Immunology, 1994, 0(3) |
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| Authors: | Liu Shi-hui Mao Pei-ji Mu Zhao-qin |
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| Abstract: | he strain of FS54-7b was an aromatic acid auxotrophic attenuated bivalent hybrid strain of S.sonnei and S.flexneri 2a constructed in our Iaboratory. This strain had been eliminated both of the tatracycline resistant gene (car-ried by Tn10)and the kanamycin resistant gene(carried by Tn5 on form I plasmid pSS120 ::Tn5).In this paper,we studied some of the biological properties of this attenuated strain in detail. The experiments proved that thisstrain was safe ,non-reverting and maintained the ability to penetrate the epithelial cells. It expressed S. sonnei andS. flexneri 2a antigens steadily,and conferred a good protection for mice against challenge with virulent S. sonnei48025-11 strain and virulent S. flexneri 2a-12 strain. |
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| Keywords: | Shigella Aromatic acid auxotrophy Attenuated strain Ability to penetrate epithelial cells Pro-tection of immunization |
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