| 流式细胞术分选在细胞膜表达Nogo-A的少突胶质细胞 |
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| 引用本文： | 熊南翔,王弛,赵洪洋,张方成,朱贤立. 流式细胞术分选在细胞膜表达Nogo-A的少突胶质细胞[J]. 医学研究生学报, 2005, 18(8): 690-691,695 |
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| 作者姓名： | 熊南翔 王弛 赵洪洋 张方成 朱贤立 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院神经外科,湖北武汉,430022
2. 华中科技大学同济医学院附属协和医院康复医学科,湖北武汉,430022 |
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| 基金项目： | 国家自然科学基金资助项目（批准号：30471775） |
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| 摘 要： | 目的：探讨成熟少突胶质细胞在细胞膜上是否有Nogo-A的表达及其比例，并收集该类活细胞。方法：不使用破膜剂，间接荧光标记细胞膜表达Nogo-A的成熟少突胶质细胞，采用流式细胞术分选被染色的阳性细胞。结果：经流式细胞术获得了细胞膜表达Nogo-A的成熟少突胶质细胞，比例为3．36％。结论：使用流式细胞技术，发现部分成熟少突胶质细胞在细胞膜表面有Nogo-A表达，其比例为3．36％。
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| 关 键 词： | Nogo-A 少突胶质细胞 流式细胞术 表达 |
| 文章编号： | 1008-8199（2005）08-0690-02 |
| 收稿时间： | 2005-02-10 |
| 修稿时间： | 2005-02-102005-02-28 |
Sorting of oligodendrocytes expressing Nogo-A in membrane by flow cytometry |
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| XIONG Nan-xiang,WANG Chi,ZHAO Hong-yang,ZHANG Fang-cheng,ZHU Xian-li. Sorting of oligodendrocytes expressing Nogo-A in membrane by flow cytometry[J]. Bulletin of Medical Postgraduate, 2005, 18(8): 690-691,695 |
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| Authors: | XIONG Nan-xiang WANG Chi ZHAO Hong-yang ZHANG Fang-cheng ZHU Xian-li |
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| Abstract: | Objective:To explore the expression of Nogo-A in membrane of mature oligodendrocyte, and obtain these living cells. Methods:Mature oligodendrocytes were stained by indirect immuno-fluorescent technique.Stained unpermeable oligodendrocytes were sorted by flow cytometry. Results: The percentage of mature oligodendrocytes with Nogo-A expression in membrane was 3.36%. Conclusion:Nogo-A could be expressed in membrane of mature oligodendrocytes,and the ratio of positive cells was about 3.36%. |
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| Keywords: | Nogo-A Oligodendrocyte Flow cytometry Expression |
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