| 胶束电动毛细管色谱分离大黄中有效成分的研究 |
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| 引用本文： | 尚小玉,袁倬斌. 胶束电动毛细管色谱分离大黄中有效成分的研究[J]. 中草药, 2001, 32(8): 691-692 |
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| 作者姓名： | 尚小玉 袁倬斌 |
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| 作者单位： | 中国科学院研究生院, |
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| 基金项目： | 国家自然科学基金(296 75022,29875027),国家"九·五"科技攻关项目(96-A23-01-06)和中科院长春应用化学研究所电分析化学开放研究实验室基金(OC 2000001)资助项目. |
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| 摘 要： | 目的 分离出大黄中4种有效成分：大黄酚、大黄素、土大黄苷和大黄酸。方法 采用胶束电动毛细管色谱法（MECC）,缓冲溶液为50mol/L H3BO3-NaOH含20mmol/L SDC ,pH值为10,分析电压17kV。结果 4种成分在5min内全部分离。结论 本法简便、快速、结果令人满意。
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| 关 键 词： | 胶束电动毛细管色谱 大黄 蒽醌类 大黄酚 大黄 土大黄苷 大黄酸 |
| 文章编号： | 0253-2670(2001)08-0691-02 |
| 收稿时间： | 2000-11-13 |
| 修稿时间： | 2000-11-13 |
Isolation of active constituents in rhubarb by micellar electrokinetic capillary chromatography |
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| SHANG Xiaoyu and YUAN Zhuobin. Isolation of active constituents in rhubarb by micellar electrokinetic capillary chromatography[J]. Chinese Traditional and Herbal Drugs, 2001, 32(8): 691-692 |
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| Authors: | SHANG Xiaoyu and YUAN Zhuobin |
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| Affiliation: | Graduate School, Chines Academy of Sciences, Beijing 100039, China;Graduate School, Chines Academy of Sciences, Beijing 100039, China |
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| Abstract: | Object To isolate and identify the four active constituents, chrysophanol, emodin, rhaponticin and rhein in rhubarb Methods Micellar electrokinetic capillary chromatography (MECC) with a buffer solution of 5 mmol/L H 3BO 3 NaOH containing 20 mmol/L of sodium deoxycholate (SDC) (pH=10) was used for their separation at a voltage of 17 kv Results The four active constituents were completely separated within 5 min Conclusion This method was found to be simple and rapid and gave satisfactory results |
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| Keywords: | micellar electrokinetic capillary chromatography (MECC) rhubarb anthraquinones |
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