HPLC法同时测定补阳还五汤中毛蕊异黄酮苷、芍药苷、羟基红花黄色素A的含量

目的 建立补阳还五汤中毛蕊异黄酮苷、芍药苷、羟基红花黄色素A的含量测定方法.方法 采用高效液相色谱法,色谱柱:Kromasil C18(250 mm×4.6 mm,5μm);流动相:甲醇-乙腈(26:2)为流动相A,0.7%磷酸为流动相B,梯度洗脱;检测波长:毛蕊异黄酮苷为260 nm,芍药苷为230 nm,羟基红花黄色素A为403 nm;柱温:35℃.结果 毛蕊异黄酮苷在0.0539～1.0780μg范围内线性关系良好,r=0.999 5,平均回收率为99.4%,RSD=1.5%;芍药苷在0.4160～8.3200μg范围内线性关系良好,r=0.999 8,平均回收率为100.6%,RSD=1.7%;羟基红花黄色素A在0.0418～0.8352μg范围内线性关系良好,r=0.999 5,平均回收率为101.0%,RSD=1.9%.结论 本方法简单快捷,适用于测定补阳还五汤中毛蕊异黄酮苷、芍药苷、羟基红花黄色素A的含量.

Simultaneous Determination of Campanulin, Paeoniflorin and Hydroxysafflor Yellow A in Buyang Huanwu Decoction by HPLC

Objective To establish a HPLC method for the simultaneous determination of campanulin, paeoniflorin and hydrox-ysafflor yellow A in Buyang Huanwu Decoction. Methods A Kromasil C18(4. 6 mm × 250 mm, 5 μm) column was adopted. The mobile phase consisited of methanol and acetonitrile(26 : 2) (A), and 0. 7 % phosphoric acid solution(B), in gradient e-lution (0～10 min, 25 % →35 % A; 10～25 min, 35 %→40 % A). The flow rate was at 1.0 mL·min-1. The column tem-perature was 35 ℃;, and the detection wavelengths were set at 260nm for campanulin, 230 nm for paeoniflorin and 403 nm for hydroxysamor yellow A. Results The linear ranges of campanuliu, paconiflorin and hydroxysafflor yellow A were 0. 0539～1. 078 μg (r=0. 999 5), O. 4160～8. 320μg (r=0. 999 8), and 0. 0418～0. 8352 μg (r=0. 999 5) respectively; the av-erage recovery was 99. 4 % (RSD=1.5 %), 100. 6 % (RSD=1.7 %), and 101.0 % (RSD=1.9 %) respectively. Conclu-sion The method is simple, feasible and reproducible, and can be used for the quality control of Buyang Huanwu Decoction.