| 结蛋白的纯化、抗体的制备及免疫组织化学鉴定 |
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| 引用本文： | 张锦生,朱虹光,孙为民,张秀荣,张月娥,应越英. 结蛋白的纯化、抗体的制备及免疫组织化学鉴定[J]. 复旦学报(医学版), 1991, 0(6) |
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| 作者姓名： | 张锦生 朱虹光 孙为民 张秀荣 张月娥 应越英 |
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| 作者单位： | 上海医科大学基础医学部病理学教研室(张锦生，朱虹光，张秀荣，张月娥)，上海医科大学88届基础医学专业班(孙为民)，上海医科大学基础医学部病理学教研室(应越英) |
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| 基金项目： | 卫生部科学研究基金(88101003) |
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| 摘 要： | 参考Small和Geisler两法加以改进。从鸡肫中提取纯化结蛋白,用淋巴结内微量注射法制备兔抗结蛋白抗体。用免疫组织化学染色法(PAP法)对抗结蛋白抗体作鉴定。结果:心肌、骨骼肌、平滑肌(除主动脉平滑肌)呈强阳性反应;胆管上皮细胞、胰腺导管上皮细胞、远端肾小管上皮细胞、汗腺分泌部上皮细胞部分呈弱阳性反应。
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| 关 键 词： | 结蛋白 纯化 抗结蛋白抗体 免疫组织化学 电泳 |
PURIFICATION OF DESMIN, PREPARATION AND IMMUNO-HISTOCHEMICAL IDENTIFICATION OF ANTI-DESMIN |
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| Zhang Jinsheng,Zhu Hongguang,Sun Weiming,Zhang Xiurong,Zhang Yuee,Ying Yueying. PURIFICATION OF DESMIN, PREPARATION AND IMMUNO-HISTOCHEMICAL IDENTIFICATION OF ANTI-DESMIN[J]. Fudan University Journal of Medical Sciences, 1991, 0(6) |
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| Authors: | Zhang Jinsheng Zhu Hongguang Sun Weiming Zhang Xiurong Zhang Yuee Ying Yueying |
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| Abstract: | Desmin was purified from chicken gizzard according to the Small and Geisler methods with some modifications. Anti-desmin was raised in rabbits by injection of micrograms of desmin into lymph nodes. Anti-desmin was identified by immu-nohistochemical staining (PAP method), the results showed that striated muscles (including myocardia, skeleton muscle) and smoth muscle (except aorta) were strongly positive. Bile duct cells, pancreatic duct cells, distal tubular cells of kidney and the duot cells of sweat glands showed weakly positive reactions sporadically. |
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| Keywords: | desmin purification anti-desmin antibody immunohisto-chemistry electrophoresis |
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