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| 怀地黄茎尖培养和植株再生技术的研究 | |
| 引用本文： | 薛建平,张爱民,李明军,谢桂英,李友勇. 怀地黄茎尖培养和植株再生技术的研究[J]. 新乡医学院学报, 2000, 17(1): 18-20 |
| 作者姓名： | 薛建平 张爱民 李明军 谢桂英 李友勇 |
| 作者单位： | 1. 新乡医学院,河南新乡,453003 2. 河南师范大学,河南新乡,453001 3. 河南职业技术师范学院,河南新乡,453003 |
| 基金项目： | 河南中医管理局攻关课题 |
| 摘 要： | 目的 研究怀地黄茎尖培养技术 ,为怀地黄茎尖脱毒苗建立最佳培养条件。方法 利用植物组织培养技术 ,将怀地黄茎尖分别在不同组合的培养基上进行培养 ,诱导其成为完整植株。结果 怀地黄茎尖在MS +6 -BA0 .3mg·L-1+NAA0 .0 2mg .L-1+GA0 .1mg·L-1培养基上能生长成健壮苗 ;在MS +PP3 3 3 1培养基上能生成健壮根系 ,长成完全小植株。结论 利用怀地黄茎尖在合适的培养基上 ,能再生出完整的植株 |
| 关 键 词： | 怀地黄 茎尖 再生植株 植物组织培养 |
| 文章编号： | 1004-7239(2000)01-0018-03 |
| 修稿时间： | 1999-11-15 |
Study on shoot - tip culture and plantlets regeneration of Rehmannia glutinosa |
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| XUE Jian-ping ,ZHANG Ai-min ,Li Ming-jun ,XIE Gui-ying ,LI You-rong. Study on shoot - tip culture and plantlets regeneration of Rehmannia glutinosa[J]. Journal of Xinxiang Medical College, 2000, 17(1): 18-20 | |
| Authors: | XUE Jian-ping ZHANG Ai-min Li Ming-jun XIE Gui-ying LI You-rong |
| Affiliation: | XUE Jian-ping 1,ZHANG Ai-min 1,Li Ming-jun 2,XIE Gui-ying 3,LI You-rong 3 |
| Abstract: | Objective The aim was studieson the effects of objective different plant hormones upon shoot- tip culture and plantlets regeneration of Rehmannia glutinosa.Method Using the biotechnology and plant tissue culture, the shoot-tip of Rehmannia glutinosa was cultured onto different media and formed regenerated plantlets.Result The shoot- tip of Rehmannia glutinosa was regenerated to form bud when it was cultured on ms basic medium with 6-BA 0.3mg·L -1 ,NAA 0.02mg·L -1 and GA 0.1mg·L -1 ;Roots could be induced when these buds were transplanted onto ms basic medium supplementedwith PP 333 1 mg·L -1 .Conclusion The shoot-tip of Rehmannia glutinosa could be regenerated to from plantlet using proper medium. |
| Keywords: | Rehmannia glutinosa shoot-tip regenerated plantlet plant tissue culture |
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