硒对小鼠高碘性氧化损伤的干预作用研究

目的:研究高碘性甲状腺肿形成的机制并寻求合适的硒干预剂量。方法:140只Balb/c小鼠分为7组:正常组、高碘组(饮水含碘3000μg/L)和5个补硒组(饮水含碘3000μg/L,硒分别为0.1、0.2、0.3、0.4、0.5mg/L),共喂养16w。放射免疫法测定甲状腺激素水平,测定血浆、肝脏、肾脏和甲状腺组织谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性,丙二醛(MDA)水平。结果:高碘组GSH-Px、SOD活性下降,MDA含量升高,而经饮水补充0.2~0.3mg/L硒组各项指标与正常组无显著性差异。结论:高碘会导致小鼠抗氧化能力下降,而经饮水补充0.2~0.3mg/L硒是有效的干预剂量。

THE INTERVENTION OF SELENIUM ON THE OXIDATIVE DAMAGE BY IODINE EXCESS IN MICE

Objective:To study the mechanism of goiter resulted from excess of iodine and seek suitable selenium intervention dosage. Method:One hundred and forty Balb/c mice were divided into seven groups: the normal control group, the high iodine group (drinking the water containing iodine 3 000μg/L) and five selenium groups (drinking the water containing iodine 3 000 μg/L and selenium 0.1, 0.2, 0.3, 0.4, 0.5mg/L). The GSH-Px、SOD activities and the concentration of MDA in plasma, liver and kidney were determined. Result:The GSH-Px、SOD activities of high-iodine group decreased significantly and MDA increased significantly compared with normal control group. There was no difference between normal control group and the groups drinking the water containing 0.2-0.3 mg/L selenium. Conclusion:Iodine excess results in oxidative damage and 0.2-0.3 mg/L selenium is an effective intervention dosage.