Development and validation of analytical method for the estimation of nateglinide in rabbit plasma |
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Authors: | Nihar Ranjan Pani Lilakant Nath Akhilesh Vikram Singh Santosh Kumar Mahapatra |
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Institution: | 1. Gayatri College of Pharmacy, At-Jamadarpali, Via-Sashan, Sambalpur, Odisha, India;2. Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India |
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Abstract: | Nateglinide has been widely used in the treatment of type-2 diabetics as an insulin secretogoga. A reliable, rapid, simple and sensitive reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for determination of nateglinide in rabbit plasma. The method was developed on Hypersil BDSC-18 column (250 mm×4.6 mm, 5 mm) using a mobile phase of 10 mM phosphate buffer (pH 2.5) and acetonitrile (35:65, v/v). The elute was monitored with the UV–vis detector at 210 nm with a flow rate of 1 mL/min. Calibration curve was linear over the concentration range of 25–2000 ng/mL. The retention times of nateglinide and internal standard (gliclazide) were 9.608 min and 11.821 min respectively. The developed RP-HPLC method can be successfully applied to the quantitative pharmacokinetic parameters determination of nateglinide in rabbit model. |
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