血浆稀释法和高速离心法消除凝血项目检测标本脂肪血干扰的实验研究

目的 研究脂肪血标本对凝血结果的干扰,并探讨血浆稀释法和高速离心法消除脂肪血标本的效果对比研究.方法 收集2017年1月～2019年12月期间健康体检者血浆标本100份(正常对照组),用脂肪乳配制成轻度脂肪血组(TG为3.955±1.053mmol/L)、中度脂肪血组(TG为8.020±3.126mmol/L)及重度脂...

Experimental Study on Eliminating Fat Blood Interference by Plasma Dilution and High Speed Centrifugation

Objective To study the interference of fat blood samples on coagulation results,and explore the effect of plasma dilution method and high-speed centrifugation method in eliminating fatty blood samples.Methods A total of 100 plasma samples(normal control group)from January 2017 to December 2019 were collected and made into mild fatty blood group(TG=3.955±1.053mmol/L),moderate fatty blood group(TG=8.020±3.126mmol/L)and severe fatty blood group(TG=22.712±5.013).The levels of triglyceride(TG),prothrombin time(PT),activated partial thromboplastin time(APTT),quantitative determination of plasma fibrinogen(FIB)and concentration of D-Dimer(DD)were detected respectively.Then 78 fatty blood samples from clinical(Study Group),including moderate fatty blood group(42cases)and severe fatty blood group(36 cases)were treated and detected by plasma dilution method and high-speed centrifugation method,and the differences among the groups were analyzed by t test.Results The levels of PT,APTT and FIB in moderate and severe fatty blood groups were higher than those in normal control group(TG<1.7mmol/L)and mild fatty blood group,and the level of DD was lower than that of normal control group and mild fatty blood group(t=-6.318~2.106,all P<0.05).78 cases of moderate and severe fatty blood samples were treated by plasma dilution method and high-speed centrifugation method,and the detection results of PT,APTT,FIB and DD between the two methods were statistically significant(t=-4.225~8.655,all P<0.05),but there was no significant difference between the two methods(all P>0.05).The linearity and precision of hemagglutination instrument were verified by plasma dilution method,and the results met the laboratory requirements.Conclusion Both plasma dilution and highspeed centrifugation can effectively eliminate the interference of moderate and severe fatty blood samples on coagulation test results.However,plasma dilution method does not need high-speed centrifugation and is simpler to operate,which avoids matrix effect and has great potential in eliminating fat blood samples.