Preparative isolation and purification of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high-speed counter-current chromatography |
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Authors: | Mei Yang Xin-Jun Xu Chun-Yan Xie Jie-Yun Huang Zhi-Sheng Xie De-Po Yang |
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Institution: | 1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China;2. Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China |
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Abstract: | An ef?cient method for the isolation and puri?cation of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high speed counter-current chromatography (HSCCC) was established in this paper. The ether extracts of Radix Eupatorii Chinensis were puri?ed by HSCCC with a solvent system of hexyl hydride–ethyl acetate–methanol–water (1:2:1:2, v/v/v/v). The upper phase was used as the stationary phase and the lower phase as the mobile phase. About 8.4 mg of 12,13-dihydroxyeuparin was obtained from 200 mg of ether extracts from Radix Eupatorii Chinensis in one-step HSCCC separation, with the purity of 96.71%, as determined by HPLC. After methanol–water recrystallization, the purity of 12,13-dihydroxyeuparin reached 99.83%. Such a simple and effective method was fairly useful to prepare pure compound as reference substances for related study on Radix Eupatorii Chinensis. |
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