Evaluation of phytochemical constituents and antioxidant activities of successive solvent extracts of leaves of Indigofera caerulea Roxb using various in vitro antioxidant assay systems |
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| Authors: | Ponmari Guruvaiah Annamalai Arunachalam Lakshmi Palanisamy Thanga Velan |
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| Institution: | 1. Department of Bioinformatics, School of Biotechnology and Health Sciences, Karunya University, Coimbatore-641 114;2. Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore-641 114;3. Centre for Bioinformatics, Pondicherry University, Puducherry-605 014;1. School of Information Technology, Indian Institute of Technology, Kharagpur, West Bengal, India;2. School of Computing Science and Engineering, VIT University, Vellore, Tamil Nadu, India;3. Department of Computer Science and Engineering, Indian Institute of Technology, Delhi, India;1. Laboratórios Integrados de Pesquisa em Ciências Químicas, Instituto de Ciências Naturais, Humanas e Sociais, Universidade Federal de Mato Grosso, Sinop, MT;2. Programa de Pós-graduação em Química, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil;1. Department of Biochemistry, Osmania University, Hyderabad, India;2. Infertility Institute and Research Centre (IIRC), Secunderabad, India;3. Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India;1. Institute of Molecular Science, Shanxi University, Taiyuan 030006, China;2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China |
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| Abstract: | ObjectiveTo evaluate the phytochemical constituents and antioxidant activities of successive solvent extracts of Indigofera caerulea Roxb using various in vitro antioxidant assay systems.MethodsTotal phenol and antioxidant activity of different solvent extracts of Indigofera caerulea Roxb leaves were investigated. Extraction was done sequentially in soxhlet apparatus using various solvents (Petroleum ether, Ethyl acetate and Methanol). Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl free radical scavenging assay, hydroxyl radical scavenging assay, superoxide anion radical scavenging assay and Total ion reducing power assay. Total phenol and flavonoid contents were also measured.ResultsMethanolic extract had more total phenol content and more antioxidant activities, confirming to the hypothesis that phenol content and antioxidant activity has a direct correlation.ConclusionsAll the results of the in vitro antioxidant assays revealed that the methanolic extract of Indigofera caerulea Roxb leaves had notable antioxidant and free radical scavenging activity. The results obtained appeared to confirm the antioxidant and free radical scavenging potential of Indigofera caerulea Roxb. |
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