CDCA5在皮肤恶性黑色素瘤中的表达及其与预后相关性的生物信息学分析

目的:探讨CDCA5在皮肤恶性黑色素瘤(cutaneous malignant melanoma,CMM)中的表达、相关信号通路及其与患者预后之间的关系.方法:应用Oncomine和GEPIA数据库研究CDCA5基因在不同肿瘤组织中的表达情况以及与患者预后关系.此外,还收集了我院41例黑色素瘤患者及41例正常皮肤病例进...

The expression of CDCA5 gene in cutaneous malignant melanoma and its correlation with prognosis of the patients by bioinformatic analysis

Objective:To explore the expression and signaling pathway of CDCA5 in cutaneous malignant melanoma(CMM)and its correlation with prognosis of CMM patients.Methods:Oncomine and TCGA databases was used to investigate the expression of CDCA5 gene in different tumor tissues.GEPIA was applied to identify the relationship between CDCA5 and prognosis of CMM patients.In addition,41 CMM and 41 normal tissues were collected for immunohistochemical(IHC)staining and evaluation.cBioPortalwas used to identify the mutation of CDCA5 in CMM.The STRING database was utilized to construct a protein-protein interaction(PPI)network,and the DAVID database and GSEA were used to perform functional enrichment and pathway analysis.TISIDB database was used to explore the relationship between CDCA5 and immune infiltration.Results:The expression level of CDCA5 gene was up-regulated in most tumor tissues,and the expression of CDCA5 in CMM was significantly higher than in normal skin tissues,which indicatedworse prognosis of CMM patients.IHC results showed increased expression of CDCA5 in CMM compared to normal tissues.Based on the data from TCGA database,8%of the CDCA5 gene was mutated in CMM.In the PPI network,the enrichment of the interacting protein network is obvious(P<1.0E-16).The molecular functions were mainly protein binding,chromosome binding,ATP binding and protein kinase activity.The biological process was mostly focused on sister chromatid cohesion,mitotic nuclear division and cell division.The KEGG signaling pathway was primarily the cell cycle,oocyte meiosis and progesterone-mediated maturation of oocytes.GSEA results showed the significantlyinvolved hallmarks were inflammation,TNFa-NF-κB pathway,interferon alpha pathway,interferon gamma pathway,IL2-STAT5 pathway,IL6-JAK-STAT3 pathway,p53 pathway and KRAS pathway.CDCA5 gene was also positively correlated with activated CD4⁺T cells,while negatively correlated with immature B cells,mast cells,type 1 helper T cells and effect memory CD8⁺T cells.Conclusion:CDCA5 is highly expressed in CMM and is closely related to the poor prognosis.