Investigation on cytotoxic,antioxidant, antimicrobial and volatile profile of Wrightia tinctoria (Roxb.) R. Br. flower used in Indian medicine |
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| Authors: | S Ramalakshmi N Edaydulla P Ramesh K Muthuchelian |
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| Institution: | 1. Department of Bioenergy, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai – 625 021, India;2. Department of Chemistry, Mother Teresa Women''s University, Kodaikanal – 624 101, India;3. Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai – 625 021, India;1. Department of Pharmacy, Banasthali University, Banasthali Rajasthan-304022, India;2. Department of Chemistry, Banasthali University, Banasthali Rajasthan-304022, India;1. Deputy for Food and Drug, Urmia University of Medical Sciences, Urmia, Iran;2. Food and Beverages Safety Research Center, Urmia University of Medical Sciences, Urmia, Iran;1. Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India;2. Department of Ocular Microbiology, Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirappalli 620 001, Tamil Nadu, India;1. School of Public Health, Jilin University, Changchun, 130021, People’s Republic of China;2. Institute of Disease Control and Prevention, Beijing, 100071, People’s Republic of China;3. College of Medicine, Shihezi University, Shihezi, People’s Republic of China;4. Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot, People’s Republic of China;5. General Hospital of People’s Armed Police Forces, Beijing, 100039, People’s Republic of China;6. Department of Orthopedics, The First Affiliated Hospital of General Hospital of People’s Liberation Army, Beijing, 100048, People’s Republic of China;7. Test Methoddology Teaching and Research Secretion, Xi’an Hygienic School, No.398 Youyi East Road, Beilin District, Xi’an, Shaanxi, 710054, People’s Republic of China;1. School of Science, Monash University Sunway Campus, Petaling Jaya, Selangor, Malaysia;2. Faculty of Applied Sciences, UCSI University, Cheras, Kuala Lumpur, Malaysia |
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| Abstract: | ObjectiveTo investigate the total phenols, flavonoids, carotenoids, antioxidant activity, antimicrobial and cytotoxic activity of Wrightia tinctoria flower extract.MethodsTotal phenols, flavonoids, carotenoids content, DPPH scavenging activity, the reducing power activity, phosphomolybednum activity, metal chelating activity, Hydrogen peroxide radical scavenging activity of crude extract, Cytotoxicity activity, GC-MS analysis and Antibacterial screening were evaluated.ResultsTotal phenols, flavonoids, carotenoids in the extract was found to be 55.29±0.45 mg GAE, 370.53±1.213 mg QE and 1.825±0.321 mg/g respectively, where the reducing power, phosphomolybednum activity and metal chelating activity were increasing with increasing concentration of the flower extract. The antioxidant activity (IC50) of the flower extract was said to be 43.16μg/mL by 2,2-Diphenyl-1-Picrylhydrazyl method and 124.07 mg AAE/100g of plant extract by phosphomolybednum method. The antibacterial studies of the ethanolic flower extract tested at different concentration of extracts, where 250mg/mL concentration of extract showed good inhibitory activity against all the test pathogens compared with standard antibiotics like streptomycin and penicillin. The cytotoxic activity of flower extract was evaluated by brine shrimp lethality bioassay method and the LC50 value found to be 3.544μg/mL.ConclusionsThe presence of major bioactive compound, hexadecanoic acid justifies the use of the whole plant for various ailments by traditional practitioners. Further studies are needed to explore the potential phenolics, flavonoid compounds from W. tinctoria for application in drug delivery, nutritional or pharmaceutical fields. |
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