基于TCGA数据库分析肝细胞肝癌组织中HMMR表达与患者临床特征及预后的相关性

目的:研究透明质酸介导的运动受体(hyaluronan-mediated motility receptor,HMMR)的表达与肝细胞肝癌(hepatocellular carcinoma,HCC)的发生发展以及预后的关系.方法:在本研究中,我们通过从癌症基因组图谱(the cancer genome atlas,TC...

The correlation between the expression of HMMR and clinical characteristics and prognosis of hepatocellular carcinoma based on TCGA database

Objective:To investigate the relationship between the expression of hyaluronan mediated motility receptor(HMMR)and the occurrence,development and prognosis of hepatocellular carcinoma(HCC).Methods:In this study,we performed bioinformatics analysis by downloading hepatocellular carcinoma-related data from the cancer genome atlas(TCGA)database,and explored the value of HMMR expression in the occurrence,development and prognosis of hepatocellular carcinoma.According to the median value of HMMR mRNA expression,the patients were divided into two groups(HMMR group with high expression and HMMR group with low expression).The relationship between HMMR expression and clinical characteristics was analyzed by Kruskal-Wallis test and Logistic regression,and the influence of HMMR expression on the survival of patients with hepatocellular carcinoma was observed by Kaplan-Meier and Cox analysis.Moreover,PPI protein interaction network,GO enrichment and KEGG pathway analysis were performed.Finally,quantitative real-time PCR(qRT-PCR)was used to verify the expression ofHMMR mRNA in HCC tissue samples.Results:The high expression of HMMR in hepatocellular carcinoma was associated with histological grade(OR=2.675 for G₃ vs G₁),clinical stage(OR=2.509 forⅢvsⅠ)and T stage(OR=3.568 for T₄ vs T₁)(all P<0.05).Kaplan-Meier survival analysis showed that HCC with HMMR high had a worse prognosis than that with HMMR-low(P=6.858E-05).Cox analysis showed that HMMR high expression was a risk factor for overall survival(OS)(HR:1.166,95%CI:1.027~1.324,P=0.018).PPI protein interactions,GO enrichment,KEGG pathway analysis,identify the interactions with HMMR 10 genes,respectively were:FAM83D,CD44,TPX2,PLK4,AURKA,PLK1,NEK2,CDK1,BUB1,DLGAP5,their main enrichment in regulation of mitotic cell cycle phase transition,cell cycle process,ECM-receptor interaction,FoxO signaling pathway and Epstein-Barr virus infection,etc.qRT-PCR results showed that HMMR mRNA was highly expressed in hepatocellular carcinoma(P<0.05).Conclusion:The high expression of HMMR mRNA is closely related to the occurrence and development of hepatocellular carcinoma,and is a risk factor for the poor prognosis of hepatocellular carcinoma.