Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma |
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Authors: | Paraag Gide Sandeep Sonawane Abhishek Chitnis |
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Institution: | 1. MET''s Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Nashik 422003, Maharashtra, India;2. Department of Clinical Sciences and Administration, University of Houston, College of Pharmacy, Houston, TX 77030, USA |
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Abstract: | A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column (250 mm×4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile:water (50:50, v/v) at flow rate of 1 mL/min. The calibration curve was linear in the range 100–3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor 1/X. |
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