抗核抗体及抗核抗体谱的实验检测方法及结果分析

目的：探讨分析实验室采用间接免疫荧光法和免疫印迹法检测ANA和ANAs的结果及价值。方法：对2317例自身抗体受检者的年龄、性别及ANA和ANAs检测结果进行分析。结果：在2317例受检者中，ANA和／或ANAs阳性914例（39．4％），女性占77．7％，男性占22．3％，36岁及以上年龄段，女性占50．1％，男性的占16．1％；ANA阳性740例（31．9％），ANAs阳性723例（31．2％），ANA 和 ANAs 均阳性549例，ANA （＋）和 ANAs （＋）符合率74.2％，ANA（－）和ANAs（－）符合率89．0％，总体符合率84．2％。结论：联合检测ANA和ANAs ，不仅可以减低假阴性率和假阳性率，而且能为诊断和鉴别诊断自身免疫病提供更有力的实验室依据。

The Analysis of Antinuclear Antibodies and Antinuclear Antibody Spectrum Assay Method and Result of Autoimmune Dis-eases

Objective ：To explore the value and results that using indirect immunofluorescence and immunoblottingto detect ANA and ANAs .Methods ：Analysis the 2 317 cases were grouped by age ,sex and the results of ANA and AN-As .Results：In this 2 317 cases ,the positive of ANA and/or ANAs were 914 cases （39 .4% ） ,women accounted for 77.7% ,males accounted for 22 .3% ,36 years and over age group ,women accounted for 50 .1% ,accounting for 16 .1%of men;ANA positive 740 cases （31 .9% ） ,ANAs tested positive for 723 cases （31 .2% ） ,ANA and ANAs were positive in 549 cases ,ANA （＋ ） and ANAs （＋ ） in line with the rate of 74 .2% ,ANA （-） and ANAs （-） compliance rate of 89 .0% ,the overall compliance rate of 84 .2% .Conclusion：Combined detection of ANA and ANAs ,not only can reduce the false-negative and false-positive rate ,but also provide the basis for more effective laboratory diagnosis and differential diagnosis of autoimmune disease .