CCL2-2518A/G单核苷酸多态性与MCP-1水平及冠心病的相关性研究

目的：探讨山东省汉族人单核细胞趋化蛋白-1（MCP-1）基因（CCL2;）启动区-2518A/G单核苷酸多态性（SNP）与血浆中MCP-1水平及冠心病患病的关系。方法：应用聚合酶链-限制性片段长度多态性（PCR-RFLP）方法检测347例冠心病患者及150例健康对照者CCL2启动子区-2518A/G SNP,应用ELISA方法检测受试者血浆中MCP-1水平。结果：经Pearsonχ2检验冠心病组与对照组等位基因频率和基因型频率存在统计学差异（P值分别为0.013和0.011）,但上述差异经冠心病危险因素校正后消失（P〉0.05）。血浆中MCP-1水平为非正态分布,经对数转换后为正态分布,进行统计学分析。AA、AG、GG基因型各组中MCP-1表达水平逐渐升高,但差异无统计学差异（F=0.560,P=0.572）。结论：CCL2-2518A/G SNP不是山东省汉族人冠心病患病的独立危险因素,且不影响血浆MCP-1表达。

Correlation between CCL2-2518A/G single nucleotide polymorphism with coronary artery disease and plasma monocyte chemoattractant protein-1

Objective：To analyze the correlation of the CCL2-2518A/G single nucleotide polymorphism with the prevalent coronary artery diseases（CAD）and plasma MCP-1level in Shandonger.Methods：A total of 347 patients with CAD and150 unrelated healthy control individuals were genotyped by polymerase chain reaction-restrict fragment length polymorphism.Plasma MCP-1concentrations of subjects were measured by enzyme linked immunosorbent assay.Results：The proportion of G allele and AG/GG genotypes were statistical significantly higher in patient group than control group by Pearsonχ2 test（P=0.013,P=0.011,respectively）,but the difference between the two groups disappeared when adjusted by conventional CAD risk factors（P〉0.05）.The plasma MCP-1concentration was skewed distribution,transformed by logarithm,the distribution became normal and was used in statistic analysis.MCP-1levels gradually increased in the AA,AG and GG genotype groups,but the difference was statistically significant（F=0.560,P=0.572）.Conclusions：CCL2-2518A/G polymorphism is not independently associated with coronary artery disease and has no influence on plasma MCP-1concentration in Shandonger.