Preparation,characterization, and cytotoxic effects of liposomal nanoparticles containing cisplatin: an in vitro study |
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| Authors: | Donya Poy Azim Akbarzadeh Hasan Ebrahimi Shahmabadi Meysam Ebrahimifar Ali Farhangi Maryam Farahnak Zarabi Azam Akbari Zahra Saffari Fatemeh Siami |
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| Institution: | 1. Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran;2. Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran;3. Department of Microbiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran;4. Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza, Iran;5. Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran |
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| Abstract: | Cisplatin is a chemotherapeutic agent used for treating various malignancies. The study aimed to prepare pegylated liposomal cisplatin and evaluate its efficacy against human breast cancer cell line MCF‐7. Drug‐loaded nanoparticles were synthesized by reverse phase evaporation technique. The study is highlighted by extensive characterization of nanoparticles in terms of nanoparticle morphology, type of drug entrapment, cisplatin retention capability, and cytotoxicity effects. The size, size distribution, and zeta potential of nanodrug were estimated 142 nm, 0.33, and ?22 mV, respectively. Drug‐loading efficiency was equal to 48% that occurred physically. Furthermore, high retention capability (39% of drug was released after 72 h) with significantly enhanced cytotoxicity of nanodrug (1.75 times more than the standard drug) confirmed the potency of liposomal nanoparticles as proper cisplatin carrier. |
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| Keywords: | atomic force microscopy cisplatin cytotoxicity drug delivery pegylated liposome |
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