结直肠癌中Cks1, P27Kip1和Skp2蛋白的表达

目的:探讨Cks1在结直肠癌发生及其在Skp2调节P27Kip1降解过程中的作用. 方法:应用流式细胞术测定正常结直肠黏膜、结直肠腺瘤和结直肠癌组织中Cks1, P27Kip1和Skp2蛋白的表达. 结果:由正常结直肠黏膜、结直肠腺瘤到结直肠癌Cks1, Skp2表达呈上升趋势(P＜0.05),P27Kip1表达呈下降趋势(P＜0.05). 结直肠癌中Cks1, Skp2表达与P27Kip1表达呈负相关(r=-0.752, P＜0.05; r=-0.746, P＜0.05);Cks1与Skp2呈正相关(r=0.845, P＜0.05). 结直肠癌中Cks1的表达与肿瘤分化程度相关(P＜0.05),而与淋巴结转移及Dukes分期不相关(P＞0.05). 结论:Cks1可能参与了结直肠癌的发生;结直肠癌中Cks1可能参与了Skp2调节P27Kip1泛素化降解过程.

Cks1, P27Kip1 and Skp2 protein expression in colorectal cancer

AIM: To investigate the expression of Cks1 protein and its relationship with the expressions of Skp2,P27~(Kip1) and colorectal tumorigenesis.METHODS: Flow cytometry was used to detect the expressions of Cks1,Skp2 and P27~(Kip1) in 20 normal colorectal mucosas,20 adenomas as well as 54 colorectal cancers.RESULTS: The expressions of Cks1 and Skp2 gradually(increased) in the order of normal mucosa,adenoma to colorectal cancer(P<0.05),while the expression of P27~(Kip1) gradually(decreased) in the same order(P<0.05).A strong correlation was found between the expressions of Cks1 and Skp2(r0.845,P<0.05) and a significant inverse relation was also observed between the expressions of Cks1 or Skp2 and of P27~(Kip1)(r-0.752,P<(0.05;) r-0.746,P<0.05 respectively).The expression of Cks1 was significantly associated with the loss of tumor differentiation(P<0.05),but not with lymph node metastasis and Dukes' staging(P>0.05).CONCLUSION: Cks1 may play an(important) role in human colorectal tumorigenesis.Cks1 may be an essential factor in proteasome-dependent degradation of P27~(Kip1) regulated by Skp2.