五种天然药物抑制晶状体上皮细胞增殖效应比较

目的：对五种天然药物姜黄素（curcumine，Cur）、榄香烯（elemene，Ele）、三氧化二砷（arsenitetrioxide，As203）、雷公藤内酯醇（triptolide，Tri）、莪术（rhizomacureumae，Re）抑制重组人碱性成纤维细胞生长因子（recombinant human basic fibroblast growth factor，rhbFGF）诱导的牛晶状体上皮细胞（1ensepithelial cell，LEC）增殖效应进行比较。方法：将rhbFGF诱导体外培养的LEC发生增殖，并用Cur、Ele、As20，、Td、Rc分别与其共同孵育后，用MTT法检测LEC的活性；用流式细胞术（flow cytometer，FCM）检测LEC增殖细胞核抗原（prolif-erating cell nuclear antigen，PcNA）表达。结果：增殖组LEC活性、PCNA蛋白表达显著高于对照组（P〈0．01）；Cur组、Ele组、As，O，组、Tri组、Rc组的LEC活性、PCNA蛋白表达均比增殖组显著降低（P〈0．01）。其中Cur、Ele的作用最强，A鄢O，的作用次之，畅作用较弱，Rc作用最弱（P〈0．01）。Cur和Ele的作用相近（P〉0．05）。结论：五种天然药物Cur、Ele、As，Q、％、Rc均可抑制LEC增殖。Cur和Ele的作用最佳，有希望成为防治后发性白内障的有效药物。

Comparison of inhibitory effect of five natural medicines on proliferation induced by rhbFGF in lens epithelial cell

AIM:To compare the inhibitory effect of five natural medicines on proliferation induced by recombinant human basic fibroblast growth factor(rhbFGF)in lens epithelial cell(LEC).METHODS:The proliferations of cultured LEC were induced by rhbFGF,which were incubated with five natural medicines,[curcumine(Cur),elemene(Ele),arsenite trioxide(As_2O_3),triptolide(Tri)and rhizoma curcumae (Rc)],respectively.The LEC activities were detected via methyl thiazolyl tetrazolium(MTT).The proliferating cell nuclear antigen(PCNA)of LEC were detected via flow cytometer(FCM).RESULTS:The activity of LEC and the expression of PCNA in proliferation group were higher than those in control group(P＜0.01).Compared with the proliferation group,the activity of LEC and the expression of PCNA were significantly decreased in group Cur,Ele,As_2O_3,Tri and Rc(P＜0.01).The inhibitory effects of Cur and Ele are the strongest.As_2O_3,in the middle.Tri and Rc are the weakest(P＜0.01),the effects of Cur and Ele are similar.CONCLUSION:The five natural medicines can inhibit the proliferation of LEC induced by rhbFGF.Cur and Ele may become effective medicines of preventing and treating after-cataract.