牡丹皮及奎尼丁对体外培养乳鼠心肌细胞动作电位的影响

实验采用细胞内微电极记录的方法,记录了体外培养心肌细胞的动作电位。并初步观察了奎尼丁及牡丹皮提取液对培养心肌细胞动作电位的影响。奎尼丁4mg/L 对培养心肌细胞呈典型的奎尼丁电生理效应,证明实验方法可靠。牡丹皮提取液对动作电位幅度、时程及 Vmax 均有抑制作用。

Effects of Quinidin and Paeonia Suffruticosa on Action Potential of Cultured Myocardial Cells

The action potential of cultured myocardial cells of newborn rats was recorded by intracellular microelectrodes.Using quinidin 4 mg/L,the amplitude of the action potential was decreased by 24.5%,while its duration was prolonged for 36.1%,and the maximum rate of depolarization during upstroke(Vmax)was decreased by 35.4%,which showed typical electrophysiological efficacy of quinidin.Using the extract of Paeonia suffruticosa 0.5 mg/ml,the amplitude,duration and Vmax of action potential were decreased 22.4%,30.1% and 27.5% respectively.These actions were progressively reduced as the concentration of Paeonia suffruticosa extract was lowered.The dosage and reaction was in positive correlation.In electrophysiological study,applying cultured myocardial cells was more advantageous than using the heart in situ or the myocardium in vitro.This report provided our results of preliminary study,and further research will be done on the pharmacology in cultured myocardial cells,on paeonol and paeonicorin,which were the active principles of Paeonia suffruticosa.