辐射对体外培养的小鼠成骨细胞核因子κB 受体活化因子配体/骨保护素mRNA表达的影响

目的 通过对成骨细胞的RANKL和OPG的基因表达分析揭示辐射对成骨细胞功能的影响.方法 在体外诱导骨髓基质细胞生成成骨细胞,碱性磷酸酶(ALP)染色对其特性进行确定,用RT-PCR方法分析了0～4 Gy照射的早期成骨细胞和成熟成骨细胞的RANKL和OPG的表达.结果 骨髓基质细胞在体外被诱导成的成骨细胞,在0～4 Gy剂量照射下,早期成骨细胞中RANKL的mRNA表达在1 Gy照射时,与0 Gy时相比表达最高,达到2.83倍,但各剂量组明显高于成熟成骨细胞的表达(t=8.34～103.57,P＜0.05).早期成骨细胞各剂量组的RANKL/OPG比值,在1 Gy照射最高达0.225±0.018,但明显高于晚期成骨细胞(t=2.84～20.99,P＜0.05).结论 辐射能够增强早期成骨细胞对破骨细胞功能的调节作用,加重骨组织的损伤.

Abstract：

Objective To study the influence of irradiation on the osteoblast function by the gene expression changes of RANKL and OPG.Methods Bone marrow stromal cells were induced to develop into early and mature osteoblasts in vitro.The characterization of osteoblasts was indentified by ALP staining.The RANKL and OPG mRNA levels in early and mature osteoblasts, which exposed to 0 -4 Gy radiation were determined by RT-PCR.Results Bone marrow stromal cells had been induced to early and mature osteoblasts by osteoblast differentiation medium in vitro.In early stage of osteoblast, RANKL mRNA expression levels treated with 1Gy irradiation was 2.83-fold higher than those other irradiation dosage groups.The RANKL mRNA expression levels of each group in early stage of osteoblasts were significantly higher than those in the mature counterpart ( t = 8.34 - 103.57, P ＜ 0.05 ).The ratio of RANKL/OPG mRNA was obviously greater in early osteoblast compared with the mature cells ( t = 2.84 - 20.99, P ＜0.05 ), and it was the highest in 1Gy irradiation treated early osteoblast.Conclusions Radiation exposure of the early osteoblasts promotes osteoclasts function and results in the bone loss.

Effect of irradiation on RANKL/OPG mRNA levels in mouse osteoblast in vitro

Objective To study the influence of irradiation on the osteoblast function by the gene expression changes of RANKL and OPG. Methods Bone marrow stromal cells were induced to develop into early and mature osteoblasts in vitro . The characterization of osteoblasts was indentified by ALP staining. The RANKL and OPG mRNA levels in early and mature osteoblasts, which exposed to 0-4 Gy radiation were determined by RT-PCR. Results Bone marrow stromal cells had been induced to early and mature osteoblasts by osteoblast differentiation medium in vitro . In early stage of osteoblast, RANKL mRNA expression levels treated with 1 Gy irradiation was 2.83-fold higher than those other irradiation dosage groups. The RANKL mRNA expression levels of each group in early stage of osteoblasts were significantly higher than those in the mature counterpart (t=8.34-103.57,P<0.05). The ratio of RANKL/OPG mRNA was obviously greater in early osteoblast compared with the mature cells(t=2.84-20.99,P<0.05), and it was the highest in 1 Gy irradiation treated early osteoblast.Conclusions Radiation exposure of the early osteoblasts promotes osteoclasts function and results in the bone loss.