| 重组人睫状神经营养因子脂质体制备及性质 |
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| 引用本文： | 高剑坤,范开,侯再金,张兵兵,杨黎,胡春兰.重组人睫状神经营养因子脂质体制备及性质[J].中国现代应用药学,2011,28(2):146-149. |
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| 作者姓名： | 高剑坤 范开 侯再金 张兵兵 杨黎 胡春兰 |
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| 作者单位： | 四川中医药高等专科学校，四川 绵阳 621000;重庆理工大学，重庆 400050；重庆富进生物医药公司，重庆 400041;四川中医药高等专科学校，四川 绵阳 621000;重庆大学生物工程学院，重庆 400044;重庆富进生物医药公司，重庆 400041;重庆富进生物医药公司，重庆 400041 |
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| 基金项目： | 绵阳市科技计划项目(08S002-4) |
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| 摘 要： | 目的制备重组人睫状神经营养因子（recombinant human ciliary neurotrophic factor,rhCNTF）脂质体,提高rhCNTF穿透血脑屏障能力。方法采用薄膜超声法制备rhCNTF脂质体,测定包封率。分别用rhCNTF原液、rhCNTF脂质体及生理盐水尾静脉注射Balb/C小鼠,0,2,6,12,24 h后测小鼠脑组织中rhCNTF含量。结果制得的rhCNTF脂质体形态圆整,平均直径为85.0 nm,包封率为93.14%。用ELISA法测定小鼠脑组织中rhCNTF的含量,rhCNTF原液组和rhCNTF脂质体组存在显著差异。结论制备的rhCNTF脂质体能提高rhCNTF穿越血脑屏障的能力,为rhCNTF的进一步应用和研究提供了依据。
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| 关 键 词： | 睫状神经营养因子 脂质体 血脑屏障 |
| 收稿时间： | 2010/5/17 0:00:00 |
Preparation and Evaluation of Liposome-encapsulated Recombinant Human Ciliary Neurotrophic Factor |
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| GAO Jiankun,FAN Kai,HOU Zaijin,ZHANG Bingbing,YANG Li and HU Chunlan.Preparation and Evaluation of Liposome-encapsulated Recombinant Human Ciliary Neurotrophic Factor[J].The Chinese Journal of Modern Applied Pharmacy,2011,28(2):146-149. |
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| Authors: | GAO Jiankun FAN Kai HOU Zaijin ZHANG Bingbing YANG Li and HU Chunlan |
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| Institution: | Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China;Chongqing University of Technology, Chongqing 400050, China; Chongqing Fagen Biomedical Inc., Chongqing 400041, China;Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China;Bioengineering College of Chongqing University, Chongqing 400044, China;Chongqing Fagen Biomedical Inc., Chongqing 400041, China;Chongqing Fagen Biomedical Inc., Chongqing 400041, China |
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| Abstract: | OBJECTIVE To enhance the penetration ability to cross the blood-brain barrier efficiently of recombinant human ciliary reurotrophic factor (rhCNTF) by encapsulated into liposome. METHODS rhCNTF liposomes were prepared by thin film dispersion-sonication method. Encapsulation efficiency was detected by HPLC. Mice were killed 0, 2, 6, 12 and 24 h after i.v. administration of rhCNTF solution, rhCNTF liposomes or saline, and then the content of rhCNTF in brain tissues was analyzed by ELISA. RESULTS rhCNTF liposomes were regular in its morphology with a mean diameter of 85.0 nm. The entrapment efficiency of rhCNTF was 93.14%. rhCNTF in mice brain tissue were different between rhCNTF solution group and rhCNTF liposomes group. CONCLUSION Liposome-encapsulated rhCNTF could efficiently cross the blood brain barrier, which provides a basis for further application and research of rhCNTF. |
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| Keywords: | ciliary neurotrophic factor liposomes blood brain barrier |
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