一氧化氮对大鼠局灶性脑缺血/再灌注后细胞死亡方式的影响

目的:研究一氧化氮(NO)对缺血性半暗带神经元死亡方式的影响。方法:在大鼠局灶性脑缺血/再灌注后不同时段上,以光镜、电镜和TUNEL法观察半暗带内细胞死亡方式,分别应用神经源性一氧化氮合酶(nNOS)、免疫源性一氧化氮合酶(i NOS)抑制剂7-硝基吲唑(7-NI)、氨基胍(AG),观察其对死亡的影响。结果:局灶性脑缺血/再灌注后半暗带内神经元死亡以凋亡方式为主,7-NI、AG可使细胞凋亡数量明显减少(方差分析P<0.05)。结论:局灶性脑缺血/再灌注半暗带内神经元死亡以凋亡为主,抑制NO的形成可减少神经元凋亡的数量。

Patterns of Cell Death Influenced by nitric oxide after Focal Cerebral IschemiaJReperfusion in Rats

Objective:To further study the patterns of cell death located in the inner boundary zone to the infarct, which was influenced by neurotoxic nitric oxide(NO).Methods:The models of different time courses after focal cerebral ischemia/reperfusion were applied to observe the patterns of cell death located in the inner boundary zone to the infarct by using lightmicroscopy, electronmicroscopy and TUNEL staining, then, inhibitors of iNOS or combined with nNOS were administered to observe their influence on the patterns of cell death.Results:The major patterns of neurons death located in the inner boundary zone to the infarct are apoptosis, and by the use of 7-NI and AG, the number of apoptotic cells markedly dropped (ANOV P<0.05).Conclusions:The patterns of cell death located in the inner boundary zone to the infarct are primarily apoptosis. If the yield of neurotoxic NO is inhibited, the number of apoptotic neurons is markedly decreased.